Fluorescence-based Cell Counter
Fluorescence-based Cell Counter - Related Videos
09:33
Determining Cell Number During Cell Culture using the Scepter Cell Counter
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Cited by 57 •
2010
The Scepter Cell Counter is a handheld automated device that can be used to count cells, monitor cell diameter and volume, and be used to check the health and quality of cellular populations from one culture to the next.
07:31
Agarose-Based Model Ecosystem for Cultivating Methanotrophs in a Methane-Oxygen Counter Gradient
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2024
A protocol is described for preparing a simple model ecosystem that recreates the methane-oxygen counter gradient found in the natural habitat of aerobic methane-oxidizing bacteria, enabling the study of their physiology in a spatially resolved context. Modifications to common biochemical assays for use with the agarose-based model ecosystem are also described.
13:16
Determination of Mammalian Cell Counts, Cell Size and Cell Health Using the Moxi Z Mini Automated Cell Counter
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Cited by 18 •
2012
The Moxi Z miniature automated cell counter is a novel instrument that combines the Coulter Principle with patented thin-film sensor technology and a proprietary software algorithm to perform sizing and counting of a broad size range of particles as well as to determine the overall health of monodisperse mammalian cell cultures. This protocol describes the use of this instrument for counting and assessing the health of cell cultures.
04:55
Electroporation-Based Delivery of Fluorescent Biomolecules into Bacterial Cells
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2025
Source: Aigrain, L., et al. Internalization and Observation of Fluorescent Biomolecules in Living Microorganisms via Electroporation. J. Vis. Exp. (2015)The video demonstrates the delivery of fluorescent biomolecules into electrocompetent microbial cells using electroporation. The process begins with the introduction of fluorophore-tagged DNA or proteins into a chilled cell suspension. A high-voltage pulse is then applied to create temporary membrane pores, enabling biomolecule entry into the...
06:57
Fluorescence-Based Quantification of Mitochondrial Membrane Potential and Superoxide Levels Using Live Imaging in HeLa Cells
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Cited by 1 •
2023
This technique describes an effective workflow to visualize and quantitatively measure mitochondrial membrane potential and superoxide levels within HeLa cells using fluorescence-based live imaging.
FAQs
The instrument illuminates the prepared sample and records emitted light from fluorescently labeled cellular components. Image analysis then distinguishes those signals from background, allowing the system to identify cell-associated fluorescence rather than treating all detected light as cellular material. This separation supports more consistent estimates of cell number and concentration.
Viability dyes add a functional distinction to fluorescence measurements: they help separate intact cells from membrane-compromised cells. This distinction allows a count to be interpreted alongside viability rather than as a total signal alone. In genetics experiments, that context helps determine whether a manipulated cell population remains suitable for subsequent work.
Reliable cell concentration measurements make starting populations more comparable across experiments. When researchers use the measured values to establish cell seeding conditions, differences in later results are less likely to arise simply from unequal numbers of cells. This consistency is especially useful when assessing genetic manipulations or comparing experimental samples.
Researchers prepare a cell sample containing the relevant fluorescent labeling and, when viability is needed, a viability dye. The instrument illuminates the sample, captures emitted light, and applies image analysis. The resulting measurements can then be used to evaluate cell number, concentration, and viability for the experimental sample.
By measuring cell number and viability after a transfection or gene-editing experiment, researchers can assess the condition of the resulting cell population and compare samples more consistently. These measurements provide population-level context for interpreting genetic manipulation outcomes, especially before molecular analyses or additional experimental steps.
Before downstream molecular analysis, measurements of cell number, concentration, and viability give researchers a quantitative basis for preparing comparable samples. This information helps identify differences in population size or condition before molecular results are interpreted. As a result, researchers can better distinguish effects associated with genetic treatment from differences in the starting cell population.
Changes in cell number can indicate that a genetic manipulation has altered the cell population, while viability measurements show whether cells remain intact or membrane-compromised. The counter does not identify the molecular cause of a change by itself, but it supplies quantitative population information that helps researchers interpret transfection or gene-editing experiments.