Hipsc Differentiation
Hipsc Differentiation - Related Videos
04:22
Differentiation of Induced Pluripotent Stem Cells into Neural Progenitor Cells
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2025
The video demonstrates a technique for differentiating human induced pluripotent stem cells (hiPSCs) into neural progenitor cells (NPCs). Initially, the hiPSCs are cultured on a feeder layer of mouse embryonic fibroblasts (MEFs). Subsequently, the cells are detached and transferred to a biopolymer-coated plate to allow the MEFs to adhere to and separate from the hiPSCs. The suspended hiPSCs are then transferred to a V-bottom plate and centrifuged to form aggregates. Finally, the addition of a...
09:30
A Guide to Generating and Using hiPSC Derived NPCs for the Study of Neurological Diseases
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Cited by 58 •
2015
This protocol describes how neural progenitor cells can be differentiated from human induced pluripotent stem cells, in order to yield a robust and replicative neural cell population, which may be used to identify the developmental pathways contributing to disease pathogenesis in many neurological disorders.
04:33
Differentiating Human Induced Pluripotent Stem Cells into Neurons on Micro-Electrode Arrays
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2025
The video demonstrates a method for generating excitatory cortical neurons from human-induced pluripotent stem cells (hiPSCs). Upon doxycycline induction, the hiPSCs overexpress neurogenin-2, a neural-specific transcription factor. By using specific media and co-culturing with astrocytes, this process facilitates the hiPSCs' differentiation into neurons with synaptic connections.
04:24
TALEN-Mediated Targeted Gene Integration: Using Engineered Sequence-Specific Nucleases for the Precise Insertion of Fluorescent Protein Gene into hiPSCs
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2025
This video describes a precise genome editing technique in human induced pluripotent stem cells, or hiPSCs, using TALENs to create double-stranded breaks at a targeted locus, inducing homology-directed repair for fluorescence protein gene integration.
06:24
Comparison of Two Representative Methods for Differentiation of Human Induced Pluripotent Stem Cells into Mesenchymal Stromal Cells
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Cited by 2 •
2023
This protocol describes and compares two representative methods for differentiating hiPSCs into mesenchymal stromal cells (MSCs). The monolayer method is characterized by lower cost, simpler operation, and easier osteogenic differentiation. The embryoid bodies (EBs) method is characterized by lower time consumption.
FAQs
Timed molecular signals guide the culture toward lineage-specific developmental pathways. As cells receive these cues, gene expression changes: pluripotency programs are silenced while genes associated with the intended cell identity become active. This coordination is central to producing specialized populations for studying how genetic differences influence cellular behavior.
Cells generated from a donor retain that individual’s genetic background, allowing researchers to examine how inherited or disease-associated variants relate to cellular phenotypes. Comparing differentiated cells with different genetic backgrounds can help connect a DNA sequence change to observable biological outcomes in a relevant human cell model.
Lineage-specific developmental pathways direct cells toward particular identities, such as neurons, cardiomyocytes, or blood cells. Their activation determines which specialized cell type emerges, whereas continued pluripotency programs would oppose that transition. Controlling these pathways therefore helps align the resulting cells with the genetic or disease question being investigated.
A protocol generally uses controlled culture conditions and timed molecular signals to guide the desired transition. The sequence regulates gene expression, reduces pluripotency-associated activity, and activates developmental programs for a selected lineage. The resulting specialized cells can then serve as the material for genetic, disease, developmental, or drug-related studies.
Researchers use this approach when they need specialized human cells that reflect a donor’s genetic background. Neurons, cardiomyocytes, blood cells, and other differentiated populations can reveal how disease-associated variants affect cellular phenotypes. This makes the system useful for linking genetic changes with biological outcomes that may not be visible in undifferentiated cells.
Differentiated cells provide specialized human model systems in which researchers can examine biological responses relevant to a disease or genetic background. These models support drug testing and can contribute to personalized medicine by preserving donor-associated genetic information while producing cell types appropriate for evaluating disease-related phenotypes and potential treatments.