Non-destructive Mounting
Non-destructive Mounting - Related Videos
08:24
Introduction to the Ultrasound Targeted Microbubble Destruction Technique
0 Views •
Cited by 19 •
2011
Ultrasound Targeted Microbubble Destruction (UTMD) can be used to direct site-specific delivery of bioactive molecules, including therapeutic genes, to target organs accessible to ultrasound, such as the heart and liver1-6.
01:12
Non-destructive Tests for Concrete Strength
0 Views •
2024
The rebound hammer test, also known as the Schmidt hammer test, is a non-destructive technique for evaluating the hardness of concrete and, indirectly, the strength of concrete. It operates on the principle that the rebound of a spring-driven mass from a concrete surface correlates to the surface's hardness. The device comprises a mass within a tubular housing, a spring mechanism, and a plunger that strikes the concrete. Upon release, the energy imparted to the mass by the spring causes it to...
10:13
Non-Destructive Evaluation of Regional Cell Density Within Tumor Aggregates Following Drug Treatment
0 Views •
Cited by 8 •
2022
The present protocol develops an image-based technique for rapid, non-destructive, and label-free regional cell density and viability measurement within 3D tumor aggregates. Findings revealed a cell-density gradient, with higher cell densities in core regions than outer layers in developing aggregates and predominantly peripheral cell death in HER2+ aggregates treated with Trastuzumab.
11:34
Subsurface Defect Localization by Structured Heating Using Laser Projected Photothermal Thermography
0 Views •
Cited by 7 •
2017
This method aims at locating vertical subsurface defects. Here, we couple a laser with a spatial light modulator and trigger its video input to heat a sample surface deterministically with two anti-phased modulated lines while acquiring highly resolved thermal images. The defect position is retrieved from evaluating thermal wave interference minima.
08:00
Whole-Mount In Situ Hybridization
0 Views •
2023
Whole-mount in situ hybridization (WMISH) is a common technique used for visualizing the location of expressed RNAs in embryos. In this process, synthetically produced RNA probes are first complementarily bound, or "hybridized," to the transcripts of target genes. Immunohistochemistry or fluorescence is then used to detect these RNA hybrids, revealing spatial and temporal patterns of gene expression. Unlike traditional in situ hybridization techniques, which require thin tissue sections whose...
FAQs
These conditions determine whether the specimen remains stable for imaging without losing recoverable structure or genetic features. Excessive pressure can distort cells or tissues, while unsuitable moisture or prolonged exposure may change the sample during observation. Careful control therefore supports reliable structural examination and increases the likelihood that the same material remains suitable for later testing.
Recoverability allows researchers to connect what they see under the microscope with later molecular or genetic testing. This is especially valuable when specimens are limited, because one preparation can support complementary investigations rather than being consumed by an irreversible step. Structural observations can consequently provide context for interpreting results obtained from the recovered material.
The key distinction is reversibility. A temporary support or mounting medium holds the material in position without permanently embedding it, cutting it, or chemically changing it. This preserves the option of removing or recovering the specimen after imaging. The approach is therefore suited to workflows that require both visual examination and subsequent genetic investigation.
The specimen is first positioned in a temporary, gentle support or mounting medium, then secured for imaging or analysis. Pressure, moisture, and exposure conditions are controlled while the relevant structures are examined. After observation, the material can be recovered for additional testing, allowing the imaging step to precede downstream molecular analysis rather than replacing it.
In genetics, the approach can support microscopy of cells, tissues, chromosomes, and other biological samples. Its value lies in preserving material for more than one type of investigation: researchers can document structural features first and then pursue molecular analysis on the recoverable specimen. The specific sample choice depends on the intended imaging and downstream testing.
It is particularly useful when a specimen is scarce or when structural and molecular information are both needed. Microscopy can reveal cellular, tissue, or chromosomal features before the material is directed to downstream genetic testing. This combined workflow conserves limited samples and helps relate visible organization to later molecular findings without requiring separate specimens for every analysis.