Sniper-screen System
Sniper-screen System - Related Videos
11:37
Using Sniper-Cas9 to Minimize Off-target Effects of CRISPR-Cas9 Without the Loss of On-target Activity Via Directed Evolution
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Cited by 21 •
2019
Here, we present a protocol to optimize CRISPR-Cas9 to achieve a higher specificity without the loss of on-target activity. We use a directed evolution approach called Sniper-screen to find a mutant Cas9 with the desired characteristics. Sniper-Cas9 is compatible with truncated single-guide RNAs and delivery in a ribonucleoprotein format, well-known strategies for achieving higher specificities.
06:47
Genome Editing in the Yellow Fever Mosquito Aedes aegypti using CRISPR-Cas9
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Cited by 2 •
2025
Here, we describe a detailed protocol for genome editing through embryonic microinjection in the mosquito A. aegypti using the CRISPR-Cas9 technology.
02:30
A CRISPR-Cas9 Technique for Gene Editing in T Cells
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2025
This video demonstrates an assay for performing gene editing in human T cells using the CRISPR-Cas9 technology. A mixture of primary CD4+ and CD8+ T cells is combined with a CRISPR-Cas9 ribonucleoprotein complex, targeting specific genes for knockout. Upon electroporation, the sgRNA guides Cas9 to the target DNA sequence, creating precise cuts. These cuts are then repaired by the cell's non-homologous end-joining mechanism, leading to gene knockout.
02:55
Genetically Modifying CAR T Cells Using a CRISPR-Cas9 System
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2025
The video outlines a process for creating genetically modified CAR T cells through the CRISPR-Cas9 System. Infecting T cells with CRISPR and CAR lentiviruses results in modifications to the target gene and the synthesis of a chimeric antigen receptor or CAR, ultimately leading to the formation of genetically modified CAR T cells.
10:07
Construction of Homozygous Mutants of Migratory Locust Using CRISPR/Cas9 Technology
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Cited by 3 •
2022
This study provides a systematically optimized procedure of CRISPR/Cas9 ribonuclease-based construction of homozygous locust mutants as well as a detailed method for cryopreservation and resuscitation of the locust eggs.
FAQs
Targeted perturbations focus the screen on selected genes or variants, allowing researchers to examine specific functional possibilities. Systematic perturbations broaden the search across a wider genetic space and can reveal candidates that were not selected in advance. This distinction affects the scope of the experiment and the type of genotype–phenotype relationships the analysis can uncover.
The phenotype provides the measurable readout that connects a genetic perturbation with a biological outcome. Selection identifies perturbations associated with a chosen outcome, while measurement can capture differences in phenotype across experiments. A clearly defined readout therefore determines which DNA changes become informative and supports comparison among candidate genes or variants.
Sequence analysis links the observed phenotype back to the DNA changes associated with the screened material. Researchers use that sequence information to identify candidate genes or variants for further interpretation. This step transforms a biological observation into genetic evidence that can support functional gene discovery and analysis of relationships between molecular changes and phenotypes.
A typical workflow begins by applying targeted or systematic genetic perturbations, followed by selecting or measuring the resulting phenotypes. Researchers then analyze associated sequence changes and connect those changes with the observed outcomes. Organizing the experiment around these stages helps maintain a clear path from perturbation, through phenotype assessment, to candidate gene or variant identification.
Researchers can apply the Sniper-screen System when they need to determine how genetic changes influence a measurable biological phenotype. It is relevant to functional gene discovery, where candidate genes are linked to outcomes, and to variant analysis, where DNA changes are examined in relation to their effects. The same framework supports larger-scale genetic investigations.
By associating molecular alterations with observable phenotypes, the system can help researchers investigate pathways underlying development or disease. Candidate genes and variants identified through screening provide points for examining how genetic changes relate to these biological processes. This makes the approach useful for connecting large-scale genetic data with pathway-level questions in genetics research.