Glomeruli Counting
Glomeruli Counting - Related Videos
03:35
Glomeruli Isolation: A Technique to Obtain Intact Glomeruli from Murine Kidney
0 Views •
2023
This video describes the method to isolate intact glomeruli from mouse kidneys. The encapsulated and decapsulated glomeruli thus obtained can further be used for culturing parietal epithelial cell outgrowths from the murine kidney.
09:50
An Efficient and Fast Method for Labeling and Analyzing Mouse Glomeruli
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2024
This study presents an easy-to-use, complete, and simple set of methods to label and analyze glomeruli from CUBIC-cleared mouse kidneys. Data such as glomerulus number and volume can be obtained easily and reliably using fluorescein isothiocyanate (FITC)-Dextran, light sheet fluorescence microscopy (LSFM), or common confocal microscopy and software such as Imaris.
12:19
Single-channel Analysis and Calcium Imaging in the Podocytes of the Freshly Isolated Glomeruli
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Cited by 25 •
2015
Changes in the intracellular calcium levels in the podocytes are one of the most important means to control the filtration function of glomeruli. Here we explain a high-throughput approach that allows detection of real-time calcium handling and single ion channels activity in the podocytes of the freshly isolated glomeruli.
10:19
Using a Hemacytometer to Count Cells
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2023
Many biomedical experiments require manipulation of a known quantity of cells, in order to achieve accurate, reproducible, and statistically-relevant data. Therefore, learning how to count cells is a particularly essential technique for any successful biomedical scientist. The most common way to count cells is by using a hemacytometer - an instrument that bears two laser-etched grids, which aid in the enumeration of an aliquot cells under a simple light microscope. This data can then be used to...
05:47
Counting and Determining the Viability of Cultured Cells
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Cited by 19 •
2008
Determining the number of cells in culture is important in standardization of culture conditions and in performing accurate quantitation experiments. In this video, we demonstrate how cells are counted using a hemacytometer.
FAQs
Glomerular density expresses the number of glomeruli relative to a measured tissue or organ context, whereas total number estimates the aggregate glomerular count. These measures answer different questions: density can indicate structural differences within tissue, while total number more directly reflects overall nephron endowment or glomerular loss. Reporting the appropriate measure improves interpretation of renal structure.
Systematic sampling helps ensure that the examined sections or regions represent the tissue or whole organ rather than a selectively chosen area. Without representative sampling, counts may overestimate or underestimate glomerular abundance because local structures can vary. This principle is especially important when investigators use tissue sections to draw conclusions about overall kidney organization or pathology.
Clear tissue preparation and consistent identification criteria are central to reliable enumeration. Investigators must distinguish glomerular profiles from surrounding structures using the visual information available in prepared sections, then apply the same criteria throughout the analysis. Consistency limits counting variation and makes comparisons between samples, developmental stages, or pathological conditions more meaningful.
Microscopy provides the visual basis for locating and recognizing glomerular profiles, while image analysis can support their quantitative enumeration. These approaches work within a broader measurement process that also requires representative sampling and consistent classification criteria. Combining visual assessment with quantitative analysis helps investigators document structural changes and report glomerular density or total number.
A typical workflow begins with preparing kidney tissue or the whole organ for examination, followed by selecting representative areas or sections. Investigators then identify glomerular profiles using consistent criteria and enumerate them through microscopy, image analysis, or both. The resulting measurements are reported as glomerular density, total number, or another clearly defined quantitative outcome.
The measurement is useful for studying nephron endowment, kidney development, and pathological remodeling. In disease-focused investigations, it can help identify glomerular loss or changes in structural organization. Relating these quantitative findings to renal function or disease progression allows researchers to connect microscopic alterations with broader medical questions about kidney health and injury.