Msc Seeding
Msc Seeding - Related Videos
04:49
MSC Seeded Scaffold Preparation: A Technique to Prepare Scaffold Seeded with Mesenchymal Stem Cells for Surgical Implantation into Murine Model
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2025
This video describes the technique of seeding mesenchymal stem cells (MSCs) onto a biodegradable poly(lactic acid) (PLA) scaffold. The prepared scaffolds can be implanted into the surgical resection cavity of the brain from where they may migrate towards tumor foci. Further, these cells can be engineered to express therapeutic proteins to stimulate apoptosis in cancer cells.
11:13
MitoCeption: Transferring Isolated Human MSC Mitochondria to Glioblastoma Stem Cells
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Cited by 51 •
2017
Here, a protocol (MitoCeption) is presented to transfer mitochondria, isolated from human mesenchymal stem cells (MSC), to glioblastoma stem cells (GSC), with the goal of studying their biological effects on GSC metabolism and functions. A similar protocol can be adapted to transfer mitochondria between other cell types.
05:06
Fluorescence-guided Tumor Resection and MSC-seeded Scaffold Implantation in Murine Model: A Surgical Procedure to Deliver Therapeutic Mesenchymal Stem Cells into Brain Tumor Resection Cavity in Mouse Model
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2025
This video presents the surgical procedure for image-guided tumor resection and implantation of a bio-scaffold seeded with genetically engineered mesenchymal stem cells or MSCs expressing tumor necrosis factor-related apoptosis-inducing ligand or TRAIL. These therapeutic MSCs have the potential in treatment of brain tumors.
11:05
Production and Administration of Therapeutic Mesenchymal Stem/Stromal Cell (MSC) Spheroids Primed in 3-D Cultures Under Xeno-free Conditions
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Cited by 21 •
2017
The therapeutic potential of mesenchymal stem/stromal cells (MSCs) is well-documented, however the best method of preparing the cells for patients remains controversial. Herein, we communicate protocols to efficiently generate and administer therapeutic spherical aggregates or 'spheroids' of MSCs primed under xeno-free conditions for experimental and clinical applications.
06:26
An Efficient Clearing Protocol for the Study of Seed Development in Tomato (Solanum lycopersicum L.)
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Cited by 2 •
2022
The tomato seed is an important model for studying genetics and developmental biology during plant reproduction. This protocol is useful for clearing tomato seeds at different developmental stages to observe the finer embryonic structure.
FAQs
Cell–surface adhesion determines whether mesenchymal stem cells remain attached after placement and can establish a stable population. Poor adhesion may reduce cellular retention and produce uneven coverage, whereas effective adhesion supports survival, proliferation, and later differentiation. Evaluating attachment is therefore important when comparing culture surfaces, biomaterials, or tissue-engineering scaffolds intended for regenerative research.
Seeding density influences how many mesenchymal stem cells initially occupy a surface or scaffold and how uniformly they are distributed. An unsuitable density can limit reproducibility or create nonuniform cellular populations, affecting subsequent proliferation and differentiation. Controlling this variable helps researchers compare engineered tissue systems more reliably and interpret differences in cellular integration.
Scaffold architecture affects where cells can attach and how evenly they occupy the available structure. Differences in architecture can therefore alter cellular distribution, attachment, survival, and proliferation within the engineered environment. Considering this variable is essential when developing biomaterial-based models, because the scaffold design can influence whether the resulting cell population integrates consistently throughout the construct.
A useful workflow controls the selected culture surface, biomaterial, or scaffold; the number of cells introduced; and the culture conditions that follow placement. Researchers then examine attachment and distribution to determine whether a viable population has been established. Maintaining consistent conditions improves reproducibility and helps distinguish effects caused by scaffold properties from those caused by cell handling.
MSC seeding is used in regenerative research to populate biomaterials and tissue-engineering scaffolds with cells capable of supporting engineered tissue development. The approach is relevant to investigations of bone, cartilage, and other damaged tissues. It also enables cell-based models in which researchers examine how cellular populations interact with engineered environments during tissue-focused studies.
Optimized MSC seeding can improve the efficiency with which cells attach and become distributed across a material or scaffold. These improvements may support greater cellular integration, more reproducible engineered tissue systems, and stronger evaluation of biomaterial-based approaches. In medical research, consistent seeding is consequently relevant to assessing the therapeutic potential of regenerative strategies.