Simultaneous Imaging

Simultaneous imaging is the coordinated acquisition of multiple images, signals, or biological features during the same observation period, helping clinicians and researchers relate complementary information in medicine. It works by synchronizing imaging systems, detector channels, or contrast signals so that structural, functional, molecular, or physiological data are captured under matched temporal conditions. This approach can reveal relationships that sequential imaging may miss, while reducing changes caused by patient movement or evolving biological processes. Applications include multimodal diagnosis, real-time monitoring of treatment responses, quantitative disease characterization, and research on dynamic processes such as blood flow, metabolism, and cellular activity.

Simultaneous Imaging - Related Videos

Research

JoVE Journal - Medicine

Simultaneous PET/MRI Imaging During Mouse Cerebral Hypoxia-ischemia

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Cited by 4 •

2015

The method presented here uses simultaneous positron emission tomography and magnetic resonance imaging. In the cerebral hypoxia-ischemia model, dynamic changes in diffusion and glucose metabolism occur during and after injury. The evolving and irreproducible damage in this model necessitates simultaneous acquisition if meaningful multi-modal imaging data are to be acquired.

Simultaneous Imaging of Microglial Dynamics and Neuronal Activity in an Awake Mouse

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2025

Source: Maruoka, H., et. al. Simultaneous Imaging of Microglial Dynamics and Neuronal Activity in Awake Mice. J. Vis. Exp. (2022) This video shows a two-photon microscopy procedure for imaging neuronal activity and microglial dynamics in an awake mouse during visual stimulation, highlighting calcium transients and cellular interactions.

Research

JoVE Journal - Neuroscience
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Simultaneous Imaging of Microglial Dynamics and Neuronal Activity in Awake Mice

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Cited by 1 •

2022

Here, we describe a protocol combining adeno-associated virus injection with cranial window implantation for simultaneous imaging of microglial dynamics and neuronal activity in awake mice.

Simultaneous Electrophysiological Recording and Calcium Imaging of Suprachiasmatic Nucleus Neurons

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Cited by 9 •

2013

Procedures are described to perform simultaneous recordings of membrane potential or current and changes of intracellular calcium concentration. Suprachiasmatic nucleus neurons are filled with the calcium indicator bis-fura-2 using a patch clamp electrode in the whole cell patch clamp configuration.

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

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Cited by 12 •

2013

We demonstrate the use of fluorescence photo activation localization microscopy (FPALM) to simultaneously image multiple types of fluorescently labeled molecules within cells. The techniques described yield the localization of thousands to hundreds of thousands of individual fluorescent labeled proteins, with a precision of tens of nanometers within single cells.

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