Councilman Chisel

The Councilman chisel is a specialized neurosurgical osteotome used to cut or remove bone during procedures involving the skull. Its beveled cutting edge concentrates force at a narrow contact area, allowing controlled separation of cranial bone when the instrument is advanced with a mallet or applied by hand, depending on its design. In neuroscience and neurosurgery, it can support craniotomy, craniectomy, and the shaping or removal of bone needed to expose intracranial structures. Proper handling helps limit unnecessary bone damage and contributes to safer surgical access, although modern powered instruments and rongeurs may be preferred for some procedures.

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Research

JoVE EoE - Assay Techniques

Ring-Shaped Micropatterning Cell Chirality Assay: An In Vitro Technique to Determine Multicellular Chirality Based on Cell Alignment on Ring-Shaped Micropatterns

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2025

In this video, we describe a cell chirality assay to determine the alignment bias of cells in a confined geometric boundary such as a ring micropattern. Chirality is an inherent property of most cell types and is determined by genetic and environmental factors. Knowing the chirality of a normal cell population can help to screen drug-treated cells.

A Micropatterning Assay for Measuring Cell Chirality

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Cited by 5 •

2022

We present a protocol for determining multicellular chirality in vitro, using the micropatterning technique. This assay allows for automatic quantification of the left-right biases of various types of cells and can be used for screening purposes.

Education

JoVE Core - Organic Chemistry

Chirality

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2023

Chirality is a term that describes the lack of mirror symmetry in an object. In other words, chiral objects cannot be superposed on their mirror images. For example, our feet are chiral, as the mirror image of the left foot, the right foot, cannot be superposed on the left foot. Chiral objects exhibit a sense of handedness when they interact with another chiral object. For example, our left foot can only fit in the left shoe and not in the right shoe. Achiral objects — objects that have...

Chirality in Nature

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2023

Chirality is the most intriguing yet essential facet of nature, governing life’s biochemical processes and precision. It can be observed from a snail shell pattern in a macroscopic world to an amino acid, the minutest building block of life. Most of the snails around the world have right-coiled shells because of the intrinsic chirality in their genes. All the amino acids present in the human body exist in an enantiomerically pure state, except for glycine - the sole achiral amino acid. The...

Molecules with Multiple Chiral Centers

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2023

Molecules that possess multiple chiral centers can afford a large number of stereoisomers. For instance, while some molecules like 2-butanol have one chiral center, defined as a tetrahedral carbon atom with four different substituents attached, several molecules like butane-2,3-diol have multiple chiral centers. A simple formula to predict the number of stereoisomers possible for a molecule with n chiral centers is 2n. However, there can be a lower number where some of the stereoisomers are...

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