Dat Mediated Current
Dat Mediated Current - Related Videos
04:27
MicroRNA-Mediated Inhibition of Excitatory Postsynaptic Currents in Mouse Hippocampal Slices
0 Views •
2025
This video demonstrates microRNA-mediated inhibition of excitatory synaptic currents in mouse hippocampal brain slices. The CA3 neurons were infected with a recombinant viral vector harboring a construct for expressing a fluorescent reporter, a light-activated membrane channel, and a microRNA that downregulates the expression of voltage-gated calcium channels. Upon placing a slice in a recording chamber and patching a CA1 neuron with a recording pipette, light pulses were used to stimulate the...
10:35
Best Current Practice for Obtaining High Quality EEG Data During Simultaneous fMRI
0 Views •
Cited by 49 •
2013
Simultaneous electroencephalography (EEG) and functional Magnetic Resonance imaging (fMRI) is a powerful neuroimaging tool. However, the inside of an MRI scanner forms a difficult environment for EEG data recording and safety must be considered whenever operating EEG equipment inside a scanner. Here, we present an optimised EEG-fMRI data acquisition protocol.
10:28
Fabrication of Nanopillar-Based Split Ring Resonators for Displacement Current Mediated Resonances in Terahertz Metamaterials
0 Views •
Cited by 1 •
2017
A protocol for the design and fabrication of a novel nanopillar-based split ring resonator (SRR) is presented.
07:28
Measurement of Bioelectric Current with a Vibrating Probe
0 Views •
Cited by 14 •
2011
The manufacture, calibration and use of non-invasive vibrating probes to measure bioelectric current in various biological systems is described.
04:20
Amphotericin-B Mediated Perforated Patch-Clamp Analysis: An Electrophysiological Technique to Study Ionic Currents in Urinary Bladder Cells
0 Views •
2023
This video demonstrates the characterization of cationic currents from transient receptor potential melastatin type 4 (TRPM4) ion channels in detrusor smooth muscle cells using amphotericin-B mediated perforated patch-clamp technique. The technique helps evaluate the biophysical and pharmacological properties of ion channels.
FAQs
Dopamine uptake through DAT is coupled to the movement of sodium and chloride, so transporter operation produces a net inward electrical signal. This coupling links the chemical process of dopamine clearance with a change in membrane excitability. Measuring the resulting current therefore provides information about both substrate transport and the electrical consequences of transporter activity.
The signal does not arise from ions flowing through an open pore, as they do in an ion channel. Instead, it accompanies the alternating transport cycle of DAT as the protein moves dopamine together with sodium and chloride. This distinction matters because the measured electrical response reflects transporter turnover and coupling, not simply channel opening or closing.
Amphetamine can drive DAT-mediated reverse transport, shifting the transporter from primarily taking up dopamine to promoting dopamine release. This mechanism changes the relationship between transporter activity and extracellular dopamine levels, helping explain how psychostimulants alter dopamine signaling. Electrophysiological measurements can be used to examine the transporter-associated electrical effects accompanying this drug action.
Electrophysiological recordings allow researchers to detect the electrical signal associated with DAT operation and evaluate how transporter activity changes under different signaling or drug conditions. The measurements can characterize transporter function, distinguish uptake-related activity from reverse transport, and clarify how dopamine handling influences membrane excitability in neuronal systems.
Because the signal connects dopamine clearance with membrane electrical behavior, it offers a functional readout of how transporter activity regulates neuronal communication. Researchers can use it to investigate the contribution of DAT to synaptic dopamine control and to examine how psychostimulants modify that control through altered transporter operation.
Changes in DAT activity can affect both dopamine availability and the electrical state of neurons. Studying the associated current therefore helps researchers examine how altered transporter function may contribute to neurological and psychiatric disorders. It also provides a framework for relating molecular transporter behavior to broader changes in dopamine signaling and drug response.