Immunocapture
Immunocapture - Related Videos
04:05
Immunocapture and Flow Cytometry of Extracellular Vesicles for Antigenic Characterization
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2025
This video demonstrates the immunocapture of extracellular vesicles using fluorescent-labeled capture antibody-magnetic nanoparticle conjugates. The process involves complex formation, staining with fluorescently labeled detection antibodies, magnetic separation, and flow cytometric analysis for antigenic characterization.
08:07
Profiling of Surface Protein Epitopes on Viral Particles by Multiplex Dual-Reporter Strategy
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2024
Here, we describe a newly developed multiplex fluorescent immunoassay that uses a dual-reporter flow cytometric system to concurrently detect two unique spike protein epitopes on intact severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) viral particles that had been captured by angiotensin-converting enzyme-2-coupled magnetic microspheres.
06:23
A Focus-Forming Assay for Quantifying Virus Titers in the Organs of an Infected Mouse
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2025
The video demonstrates a procedure for quantifying the Zika virus in multiple mouse organs via a focus-forming assay. The process involves infecting mammalian cell monolayers with virus-infected tissue homogenates, followed by the use of methyl cellulose to prevent viral spread. Subsequently, immunostaining is employed to enable precise quantification and measurement of organ-specific infectivity.
FAQs
Antibody specificity determines which molecular target becomes enriched from a complex sample. An antibody that recognizes the intended protein, particle, or cell allows the captured material to represent that target rather than unrelated sample components. In neuroscience, this selectivity is important when examining synaptic proteins, receptors, extracellular vesicles, or disease-associated molecules with downstream detection methods.
Washing removes material that has not been specifically retained by the antibody-supported matrix, helping reduce unwanted components in the isolated fraction. Elution then releases the captured target for detection or further analysis. Together, these steps determine whether the final preparation is sufficiently concentrated and clean to support interpretation of molecular findings.
The approach can be applied to proteins, particles, and cells, so its usefulness extends across several levels of neuroscience investigation. Examples include synaptic proteins, receptors, extracellular vesicles, and disease-associated molecules. This range allows researchers to examine neuronal signaling components as well as molecular material linked with neurodegenerative disease mechanisms.
A typical workflow begins by immobilizing an antibody on a solid support, such as magnetic beads, and exposing that support to a complex biological sample. The target binds to the antibody, unbound material is removed by washing, and the retained material is eluted or taken directly into a downstream detection step.
Brain tissue can provide access to molecular components located within neural structures, including synaptic proteins and receptors. Biofluids can be used to examine extracellular vesicles or disease-associated molecules present outside the tissue. Choosing between these sample contexts depends on whether the study focuses on neuronal components, accessible molecular signals, or biomarker discovery.
The isolated material can support investigations of neuronal signaling, biomarker discovery, and mechanisms associated with neurodegenerative disease. Enriching a selected target concentrates it for downstream detection, making molecular features easier to examine within a complex sample. The resulting data can therefore connect specific proteins, particles, or cells with broader disease or signaling questions.