Stimulus Isolation Unit

A stimulus isolation unit is a neuroscience device that electrically separates a stimulation circuit from ground while delivering controlled pulses to neural tissue, improving experimental safety and signal reliability. It receives timing signals from a stimulator and uses electrical isolation to transfer the stimulus without a direct conductive path, helping limit ground loops, unwanted current, and interference. Researchers use these units in electrophysiology, neurophysiology, and behavioral experiments to apply reproducible electrical stimulation to nerves, brain regions, or muscles while recording neural activity. This isolation supports cleaner measurements and helps protect both biological preparations and connected recording equipment.

Stimulus Isolation Unit - Related Videos

Research

JoVE EoE - Neuronal Culture Techniques

A Method for the Isolation of Neurovascular Units from Rat Brain

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2025

This video demonstrates the process of isolating brain microvessels from euthanized rats. It includes the dissection of the brain, cleaning of the tissue, and mechanical digestion, followed by repeated centrifugation to isolate the pure fraction of microvessels.

Research

JoVE Journal - Developmental Biology

Identification and Isolation of Burst-Forming Unit and Colony-Forming Unit Erythroid Progenitors from Mouse Tissue by Flow Cytometry

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Cited by 13 •

2022

Here, we describe a novel flow cytometric method for prospective isolation of early burst-forming unit erythroid (BFU-e) and colony-forming unit erythroid (CFU-e) progenitors directly from fresh mouse bone marrow and spleen. This protocol, developed based on single-cell transcriptomic data, is the first to isolate all the tissue's erythroid progenitors with high purity.

Inducing Axonal Varicosities with a Micromechanical Stimulus on Neurons

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2025

This video demonstrates the induction of axonal varicosities by applying micromechanical stimulus on neurons. The system comprises a micropipette attached to a syringe containing buffer via tubing. Using a micromanipulator, the micropipette is positioned above the neurons. Fluid pressure is then applied to induce varicosities in axons.

Isolation of Murine Adipose Tissue-derived Microvascular Fragments as Vascularization Units for Tissue Engineering

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Cited by 59 •

2017

We present a protocol to isolate adipose tissue-derived microvascular fragments that represent promising vascularization units. They can be rapidly isolated, do not require in vitro processing and, thus, may be used for one-step prevascularization in different fields of tissue engineering.

Education

JoVE Core - Chemistry

Measurement: Standard Units

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2020

Every measurement provides three kinds of information: the size or magnitude of the measurement (a number), a standard of comparison for the measurement (a unit), and an indication of the uncertainty of the measurement. While the number and unit are explicitly represented when a quantity is written, the uncertainty is an aspect of the errors in the measurement results. The number in the measurement can be represented in different ways, including decimal form and scientific notation, which is...

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