Systemic Injection
Systemic Injection - Related Videos
02:34
Intraperitoneal Injection of Bacterial Lipopolysaccharide in Mice to Study Systemic Immune Responses
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2026
Source: Radulovic, K., et al. Injections of Lipopolysaccharide into Mice to Mimic Entrance of Microbial-derived Products After Intestinal Barrier Breach. J. Vis. Exp. (2018)This video demonstrates a step-by-step procedure for injecting lipopolysaccharide (LPS) into mice intraperitoneally to induce systemic inflammation.
09:24
Systemic Injection of Neural Stem/Progenitor Cells in Mice with Chronic EAE
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Cited by 19 •
2014
The transplantation of neural stem/progenitor cells (NPCs) holds great promises in regenerative neurology. The systemic delivery of NPCs has turned into effective, low invasive, and therapeutically very efficacious protocol to deliver stem cells in the brain and spinal cord of rodents and nonhuman primates affected by experimental chronic inflammatory damage of the central nervous system.
03:52
Subcutaneous Injection of Bacterial Antigen to Induce Systemic Inflammation in a Murine Model
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2025
This video demonstrates the induction of systemic inflammation in an anesthetized mouse. The injection of mycobacterial antigen-adjuvant depots triggers cytokine release and localized inflammation. Lymphatic vessels open, enabling immune cell entry into lymph nodes and activation of T cells, which release pro-inflammatory cytokines, leading to widespread inflammation.
01:08
Gas Chromatography: Sample Injection Systems
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2024
In gas chromatography, the sample is introduced as a vapor plug into the carrier gas stream for high efficiency and resolution. A microsyringe injects the sample solution into a heated sample port, vaporizing it and mixing it with the carrier gas. This process is important to ensure the sample is properly prepared for analysis. Thermally sensitive samples can be injected directly into the column and volatilized by slowly increasing the column temperature. Two primary injection methods are used...
02:39
Systemic Treatment for Postnatal, Juvenile, and Runted Adult Mice by Retrobulbar Sinus Injection
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2024
This article provides a protocol and an accompanying video for the retrobulbar sinus injection of up to a total volume of 150 µL for postnatal, juvenile, and runted adult mice. This procedure is particularly well suited for the injection of small mice (15 g) when tail vein injection is not feasible.
FAQs
These routes produce different absorption rates and distribution patterns after administration. Consequently, the timing and extent of exposure across organs can vary even when the same compound is used. Recognizing these route-dependent differences helps researchers interpret neural, behavioral, and physiological measurements and select conditions that support reproducible whole-body experiments.
Dose influences how much compound becomes available to the body, while timing determines when researchers assess its effects. Controlling both variables makes results more comparable between experiments and helps relate a measured brain, behavioral, or physiological response to the administered treatment rather than to inconsistent exposure conditions.
Brain effects depend partly on how the compound is distributed through the circulation and whether it can interact with or cross the blood-brain barrier. A compound may therefore produce broad physiological effects without producing the same degree of neural exposure. Considering this barrier is essential when interpreting brain responses after administration.
Because administration routes differ in absorption and distribution, they can shape how a compound reaches multiple organs and when effects become measurable. Researchers must account for the selected route when comparing neural, behavioral, or physiological outcomes. This context helps distinguish the observed response pattern from a route-independent conclusion about the compound.
A study begins by selecting the compound and an appropriate route, then establishing the dose and timing of administration. After delivery, researchers measure relevant neural, behavioral, or physiological outcomes. Keeping these variables controlled across experiments supports reproducibility and improves interpretation of how the treatment relates to observed whole-body or brain responses.
The approach can deliver drugs, hormones, tracers, and other agents, depending on the experimental question. These materials may be used to examine pharmacological effects, physiological signaling, distribution, or neural responses. Identifying the agent’s intended measurement helps determine which outcomes, such as behavior or brain activity, should be evaluated.
It is particularly useful when investigators need to examine how a treatment influences the nervous system alongside other organs and physiological processes. Animal and clinical studies can use the approach to measure neural, behavioral, or physiological outcomes, making it relevant for connecting brain responses with broader effects throughout the body.