Turtle Head Isolation

Turtle head isolation is a specialized neuroscience preparation that separates a turtle’s head from the body to provide direct access to the brain while preserving neural structures for laboratory study. The procedure involves carefully isolating the head and maintaining the exposed tissue under controlled physiological conditions, allowing researchers to examine neural activity, anatomy, and responses with reduced interference from peripheral systems. This preparation supports investigations of sensory processing, motor circuits, brainstem function, and neural connectivity. By enabling focused experiments on intact or minimally disturbed central nervous tissue, turtle head isolation can help link cellular mechanisms with the organization and function of vertebrate neural systems.

Turtle Head Isolation - Related Videos

Research

JoVE Journal - Developmental Biology
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Isolation and Characterization of Satellite Cells from Rat Head Branchiomeric Muscles

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Cited by 10 •

2015

This protocol describes the isolation of satellite cells from branchiomeric head muscles of a 9 week-old rat. The muscles originate from different branchial arches. Subsequently, the satellite cells are cultured on a spot coating of millimeter size to study their differentiation. This approach avoids the expansion and passaging of satellite cells.

Research

JoVE EoE - Neuronal Culture Techniques

A Technique to Isolate Satellite Cells From Rat Head Muscle Tissue

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2025

This video demonstrates the isolation of satellite cells from rat head branchiomeric muscle tissues through tissue dissection, enzymatic digestion, and subsequent mechanical and centrifugal purification. The cells are then seeded on extracellular matrix-coated wells for differentiation into myoblasts, and eventually into myotubes and mature muscle fibers.

Research

JoVE Journal - Neuroscience

Head Implants for the Neuroimaging of Awake, Head-Fixed Rats

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2022

A detailed new procedure for functional imaging of awake, head-fixed rats is described.

Isolation, Fixation and Characterization of Juvenile Gilthead Seabream Head Kidney Leukocytes by Flow Cytometry

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Cited by 1 •

2025

This manuscript describes the isolation and fixation of leukocytes extracted from gilthead seabream's head-kidney and the assessment of their viability by flow cytometry. This work contributes to the standardization of protocols and leverages the processing of a higher number of samples without compromising sample quality, promoting advancements in fish immunology knowledge.

Application of Passive Head Motion to Generate Defined Accelerations at the Heads of Rodents

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Cited by 1 •

2022

The present protocol describes a custom-designed ''passive head motion'' system, which reproduces mechanical accelerations at rodents' heads generated during their treadmill running at moderate velocities. It allows dissecting mechanical factors/elements from the beneficial effects of physical exercise.

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