Vsv Pseudotyping
Vsv Pseudotyping - Related Videos
11:08
Packaging HIV- or FIV-based Lentivector Expression Constructs & Transduction of VSV-G Pseudotyped Viral Particles
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Cited by 16 •
2012
Lentiviral expression vectors are the most effective vehicles for stably expressing different effector molecules or reporter constructs in dividing and non-dividing mammalian cells and whole organisms. Here we provide a protocol on how to package lentivector expression constructs in pseudoviral particles and to transduce target cells using the pseudoviral particles.
05:49
Pseudotyped Viruses As a Molecular Tool to Monitor Humoral Immune Responses Against SARS-CoV-2 Via Neutralization Assay
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2023
Pseudotyped viruses (PVs) are replication-defective virions that are used to study host-virus interactions under safer conditions than handling authentic viruses. Presented here is a detailed protocol that shows how SARS-CoV-2 PVs can be used to test the neutralizing ability of patients' serum after COVID-19 vaccination.
08:10
Production of High-Titer Infectious Influenza Pseudotyped Particles with Envelope Glycoproteins from Highly Pathogenic H5N1 and Avian H7N9 Viruses
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Cited by 3 •
2020
This protocol describes an experimental process to produce high-titer infectious viral pseudotyped particles (pp) with envelope glycoproteins from two influenza A strains and how to determine their infectivity. This protocol is highly adaptable to develop pps of any other type of enveloped viruses with different envelope glycoproteins.
08:40
Production of Pseudotyped Particles to Study Highly Pathogenic Coronaviruses in a Biosafety Level 2 Setting
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Cited by 89 •
2019
Here, we present a protocol to generate pseudotyped particles in a BSL-2 setting incorporating the spike protein of the highly pathogenic viruses Middle East respiratory syndrome and severe acute respiratory syndrome coronaviruses. These pseudotyped particles contain a luciferase reporter gene allowing quantification of virus entry into target host cells.
10:10
Retroviral Scanning: Mapping MLV Integration Sites to Define Cell-specific Regulatory Regions
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2017
Here, we describe a protocol for genome-wide mapping of the integration sites of Moloney murine leukemia virus-based retroviral vectors in human cells.
FAQs
The supplied envelope glycoprotein determines which receptors a particle can use and therefore influences which cells it can enter. During production, researchers replace or functionally remove VSV glycoprotein G and provide a selected heterologous glycoprotein instead. This separates the particle’s entry behavior from its retained VSV-based gene-delivery machinery, enabling entry properties to be studied or exploited experimentally.
Changing the donor glycoprotein changes receptor usage and cellular tropism, meaning the range or identity of cells that can be entered. VSV Pseudotyping therefore provides a way to compare how different envelope proteins redirect the same VSV-based delivery framework. In neuroscience, this distinction is useful when investigators need controlled cell-type targeting without changing the underlying gene-delivery platform.
Retaining VSV-based gene-delivery machinery allows engineered particles to carry genetic payloads while the substituted envelope controls entry. This division of functions is important because it links where a particle can enter with what genetic material it delivers. Researchers can consequently examine how entry properties affect neural experiments without treating the envelope and payload-delivery roles as the same feature.
Production requires removing or functionally replacing the native VSV glycoprotein G and supplying a selected heterologous glycoprotein during particle production. The resulting particles preserve VSV-based gene-delivery machinery while acquiring entry properties associated with the supplied glycoprotein. This workflow creates particles whose cellular access can be adjusted through the chosen envelope component.
Replication-defective particles can improve experimental control by emphasizing genetic delivery without ongoing viral propagation. In neuroscience, this constraint helps investigators interpret targeting and delivery outcomes within a bounded experimental design, rather than conflating those outcomes with unrestricted replication. Such control is especially relevant when researchers connect particle entry properties to neural function or circuit organization.
In neuroscience, engineered particles support controlled delivery of genetic payloads, cell-type targeting, and circuit tracing. Their altered entry properties can help investigators study neuronal connectivity by directing delivery according to the selected glycoprotein’s receptor usage and tropism. These applications also allow researchers to relate viral entry behavior to neural function while preserving a VSV-based delivery framework.