Laser Micro Machining

Laser micromachining is a precision manufacturing process that uses focused laser energy to cut, drill, pattern, or modify materials at micrometer-scale dimensions. The laser concentrates light into a small region, where controlled energy deposition causes localized melting, vaporization, ablation, or other material changes; beam position, intensity, wavelength, and pulse duration determine the result. In physics and engineering, the technique supports fabrication of microchannels, apertures, sensors, electronic components, and intricate surface structures across metals, polymers, ceramics, and other materials. Its noncontact operation and fine spatial control enable complex geometries, reduced mechanical stress, and rapid prototyping for research and advanced manufacturing.

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Research

JoVE Journal - Biology
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Laser Micro-Irradiation to Study DNA Recruitment During S Phase

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Cited by 4 •

2021

This protocol describes a non-invasive method to efficiently identify S-phase cells for downstream microscopy studies, such as measuring DNA repair protein recruitment by laser micro-irradiation.

Quantitative Locomotion Study of Freely Swimming Micro-organisms Using Laser Diffraction

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Cited by 10 •

2012

Microscopic organisms like the free-swimming nematode C. elegans, live and behave in a complex three-dimensional environment. We report on a novel approach that provides analysis of C. elegans using diffraction patterns. This approach consists of tracking the temporal periodicity of diffraction patterns generated by directing laser light through a cuvette.

Application of Laser Micro-irradiation for Examination of Single and Double Strand Break Repair in Mammalian Cells

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Cited by 27 •

2017

Confocal fluorescence microscopy and laser micro-irradiation offer tools for inducing DNA damage and monitoring the response of DNA repair proteins in selected sub-nuclear areas. This technique has significantly advanced our knowledge of damage detection, signaling, and recruitment. This manuscript demonstrates these technologies to examine single and double strand break repair.

Education

JoVE Science Education - Engineering

AC Synchronous Machine Synchronization

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2023

Source: Ali Bazzi, Department of Electrical Engineering, University of Connecticut, Storrs, CT. Three-phase wound-rotor synchronous generators are the main source of electrical power worldwide. They require a prime mover and an exciter in order to generate power. The prime mover can be a turbine spun by fluid (gas or liquid), thus the sources of the fluid can be water running off a dam through a long nozzle, steam from water evaporated using burned coal, etc. Most power plants including coal,...

AC Synchronous Machine Characterization

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2023

Source: Ali Bazzi, Department of Electrical Engineering, University of Connecticut, Storrs, CT. Three-phase wound-rotor synchronous motors are less popular than permanent magnet rotor synchronous motors due to the brushes required for the rotor field. Synchronous generators are much more common and available in most existing power plants, as they have excellent frequency and voltage regulation. Synchronous motors have the advantage of almost 0% speed regulation due to the fact that the rotor...

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