Photogate Timer

A photogate timer is an electronic device that measures the timing of moving objects by detecting when they interrupt a beam of light, making precise motion analysis possible in physics experiments. Typically, a light-emitting diode and photodetector form a beam across a track; when a cart, pendulum, or opaque flag blocks or restores the beam, the timer records one or more transition times, which can be combined with known distances or flag lengths to calculate speed, acceleration, or period. Photogate timers reduce reaction-time errors and support quantitative studies of motion, collisions, free fall, and oscillations.

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JoVE EoE - Antibody-Based Technologies

An Assay to Measure Influenza Neuraminidase Inhibition Antibody Titers

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2025

This video demonstrates an assay measuring influenza neuraminidase (NA) inhibiting antibody titers in a serum sample. A serum sample containing antibodies against NA is added to a fetuin-coated microwell plate. Upon adding the virus, the antibodies prevent viral NA from cleaving fetuin and exposing its galactose moiety. A peroxidase-conjugated lectin is then added, which binds to the exposed galactose. The NA-inhibiting antibody titer is determined using a colorimetric test, where a peroxidase...

Assessing Lentiviral Titer Using an Enzyme-Linked Immunosorbent Assay

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2026

Source: Vijayraghavan, S., et al. Protocol for the Production of Integrase-deficient Lentiviral Vectors for CRISPR/Cas9-mediated Gene Knockout in Dividing Cells. J. Vis. Exp. (2017).This video demonstrates the use of a p24 ELISA to estimate viral titers of lentiviral vectors. The assay involves lysing viral particles to release p24, capturing it with specific antibodies, and detecting it with a peroxidase-linked system that produces a measurable color change. By comparing sample absorbance...

An Optimized Hemagglutination Inhibition (HI) Assay to Quantify Influenza-specific Antibody Titers

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Cited by 82 •

2017

The presented protocols describe how to perform a hemagglutination inhibition assay to quantify influenza-specific antibody titers from serum samples of influenza vaccine recipients. The first assay determines optimal viral antigen concentrations by hemagglutination. The second assay quantifies influenza-specific antibody titers by hemagglutination inhibition.

Research

JoVE Journal - Immunology and Infection
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Treatment of Platelet Products with Riboflavin and UV Light: Effectiveness Against High Titer Bacterial Contamination

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Cited by 19 •

2015

The storage conditions of platelet products create an ideal environment for bacterial contaminants to multiply to dangerous levels. The goal of this study was to use a colony forming assay to evaluate a riboflavin based pathogen reduction process against high titer bacterial contamination in human platelet products.

A Microneutralization Assay to Measure Neutralizing Antibody Titers in Human Serum

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2025

This video demonstrates Microneutralization assays to measure neutralizing antibodies using Madin-Darby Canine Kidney cells overexpressing α2,6-linked sialic acid in human sera. This assay is often used for influenza human...

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