N Minus One

N minus one (n−1) is a statistical quantity commonly used to represent the degrees of freedom available when estimating variability from a sample. After calculating the sample mean, one constraint links the observations because their deviations from that mean must sum to zero, leaving only n−1 independent deviations; this principle motivates the n−1 denominator in sample variance and standard deviation. Using n−1 rather than n applies Bessel’s correction, reducing the tendency of sample variance to underestimate population variability. The concept supports reliable estimation, hypothesis testing, confidence intervals, and other statistical analyses based on sampled data.

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Research

JoVE Journal - Medicine

Electrocardiogram Recordings in Anesthetized Mice using Lead II

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Cited by 7 •

2020

We present an ECG protocol that is technically easy, inexpensive, fast, and affordable in small mice, and can be performed with enhanced sensitivity. We suggest this method as a screening approach for studying pharmacological agents, genetic modifications, and disease models in mice.

Stimulated Stokes and Antistokes Raman Scattering in Microspherical Whispering Gallery Mode Resonators

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Cited by 2 •

2016

Efficient generation of nonlinear phenomena related to third order optical non-linear susceptibility Χ(3) interactions in triply resonant silica microspheres is presented in this paper. The interactions here reported are: Stimulated Raman Scattering (SRS), and four wave mixing processes comprising Stimulated Anti-stokes Raman Scattering (SARS).

Research

JoVE Journal - Environment
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Spotting Cheetahs: Identifying Individuals by Their Footprints

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Cited by 26 •

2016

The cheetah (Acinonyx jubatus) is an iconic, endangered species, but conservation efforts are challenged by habitat shrinkage and conflict with commercial farmers. The footprint identification technique, a robust, accurate and cost-effective image classification system, is a new approach to monitoring cheetahs.

Generation of Induced Pluripotent Stem Cells from Human Peripheral T Cells Using Sendai Virus in Feeder-free Conditions

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Cited by 11 •

2015

This protocol describes how to generate induced pluripotent stem cells (iPSCs) from human peripheral T cells in feeder-free conditions using a combination of matrigel and Sendai virus vectors containing reprogramming factors.

Using Terminal Transferase-mediated dUTP Nick End-labelling (TUNEL) and Caspase 3/7 Assays to Measure Epidermal Cell Death in Frogs with Chytridiomycosis

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Cited by 3 •

2018

We quantify epidermal cell death in frogs with chytridiomycosis using two methods. First, we use terminal transferase-mediated dUTP nick end-labelling (TUNEL) in situ histology to determine differences between clinically infected and uninfected animals. Second, we conduct a time series analysis of apoptosis over infection using a caspase 3/7 protein analysis.

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