Thin Disc
Thin Disc - Related Videos
07:48
Dissection and Mounting of Drosophila Pupal Eye Discs
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Cited by 6 •
2014
The goal of this technique is to enable researchers to perform dissection, immunostaining and mounting of pupal eye discs from Drosophila melanogaster of any age.
06:08
Long-term Live Imaging of Drosophila Eye Disc
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Cited by 6 •
2017
This protocol describes a detailed method for the long-term ex vivo culture and live imaging of a Drosophila imaginal disc. It demonstrates photoreceptor differentiation and ommatidial rotation within the 10 h period of live imaging of the eye disc. The protocol is simple and does not require expensive setup.
07:36
Dissection of Imaginal Discs from 3rd Instar Drosophila Larvae
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Cited by 10 •
2007
This protocol demonstrates the dissection technique used for isolating imaginal discs from drosophila larvae at the 3rd instar stage. Methods for fixing the tissue after saying and removing the wing, leg, and eye discs are demonstrated directly on a microscope slide for subsequent visualization.
08:03
An In Vitro Organ Culture Model of the Murine Intervertebral Disc
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Cited by 22 •
2017
Whole organ culture of the intervertebral disc (IVD) preserves the native extracellular matrix, cell phenotypes, and cellular-matrix interactions. Here we describe an IVD culture system using mouse lumbar and caudal IVDs in their functional spinal units and several applications utilizing this system.
06:07
A Novel RFP Reporter to Aid in the Visualization of the Eye Imaginal Disc in Drosophila
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Cited by 1 •
2009
We describe a novel red fluorescent protein (RFP) reporter that is expressed specifically in the Drosophila eye. We detail a methodology for dissection of the eye imaginal disc and how this reporter can be used to aid in the dissection and identification of specific cell types in the developing eye.
FAQs
Radial stress acts along lines extending from the center toward the rim, while circumferential stress acts around the disc. Applied loads and rotation generate these stress components together, and their distribution depends on the disc’s geometry, material properties, and support conditions. Evaluating both is essential for assessing strength, deformation, and possible failure in rotating or pressure-loaded components.
Because the thickness is small compared with the diameter, engineers can often simplify the analysis by treating the dominant stresses as acting within the disc’s plane. This reduces the complexity of predicting deformation and strength while retaining the important radial and circumferential effects. The approximation supports efficient evaluation of lightweight structures and rotating machinery.
Deformation depends on more than the applied load. Material properties govern how readily the disc responds, while geometry affects the structural distribution of stress and stiffness. Support conditions also change the response by restricting or permitting movement. Rotation introduces another important loading condition, so engineers must consider material, dimensions, supports, and loading together when predicting performance.
An analysis begins by specifying the disc’s material properties, geometry, support conditions, and loading. The engineer then evaluates the resulting radial and circumferential stresses and the associated deformation, using the plane-stress approximation when appropriate. These results can be used to judge strength, stiffness, vibration behavior, and potential failure, rather than relying on geometry alone.
Engineers apply this analysis to components such as flywheels, turbine rotors, and brake discs, where rotation can produce significant structural stresses and deformation. The calculations help determine whether the component can maintain adequate strength and stiffness during operation. They also support designs that use material efficiently while targeting safer and higher-performance mechanical systems.
For pressure-loaded circular plates, the model relates loading and support conditions to radial and circumferential stresses and resulting deformation. In lightweight structures, the same approach helps engineers examine how reduced material affects stiffness and strength. The resulting predictions can guide safer designs, efficient material use, and evaluation of failure risks in circular components.