Murine Heart
Murine Heart - Related Videos
08:12
Intramyocardial Cell Delivery: Observations in Murine Hearts
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Cited by 9 •
2014
Intramyocardial cell delivery in murine models of cardiovascular diseases, such as hypertension or myocardial infarction, is widely used to test the therapeutic potential of different cell types in regenerative studies. Therefore, a detailed description and a clear visualization of this surgical procedure will help to define the limits and advantages of cardiovascular cell therapeutic analyses in small rodents.
10:27
Murine Heterotopic Heart Transplant Technique
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Cited by 18 •
2014
The manuscript describes the steps required to perform the heterotopic heart transplant in the mouse.
07:51
Murine Short Axis Ventricular Heart Slices for Electrophysiological Studies
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Cited by 3 •
2017
Here, we describe the preparation of viable ventricular slices from adult mice and their use for sharp electrode action potential recordings. These multicellular preparations provide a preserved in vivo like tissue structure, which makes them a valuable model for electrophysiological and pharmacological studies in vitro.
07:49
A Pacing-Controlled Procedure for the Assessment of Heart Rate-Dependent Diastolic Functions in Murine Heart Failure Models
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2023
The present protocol describes obtaining the pressure-volume relationship through transesophageal pacing, which serves as a valuable tool in evaluating diastolic function in mouse models of heart failure.
08:23
Modified Technique for the Use of Neonatal Murine Hearts in the Langendorff Preparation
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Cited by 1 •
2022
The present protocol describes aortic cannulation and retrograde perfusion of the ex-vivo neonatal murine heart. A two-person strategy, using a dissecting microscope and a blunted small gauge needle, permits reliable cannulation. Quantification of longitudinal contractile tension is achieved using a force transducer connected to the apex of the left ventricle.
FAQs
Electrical conduction coordinates activation across the chambers, while calcium regulation links that activation to cardiac muscle contraction. Together, these processes determine how an engineered intervention may influence beating and mechanical performance. Measuring physiological responses in this setting helps researchers identify whether a biomaterial or tissue-engineered construct supports coordinated function rather than producing an isolated structural change.
A cardiac construct must be considered within the coordinated activity of the four chambers and the direction imposed by the valves. These features connect tissue behavior with circulation, so assessment can extend beyond cell or material compatibility. In murine-heart studies, researchers can examine whether an intervention alters myocardial mechanics, flow-related performance, or overall heart function.
Measurable physiological responses provide functional evidence that complements structural observations. Changes in myocardial mechanics, coordinated contraction, circulation, or overall heart performance can indicate how a material, construct, or delivery system interacts with the organ. This combined perspective is useful because a promising design must influence cardiac behavior appropriately, not merely occupy or contact heart tissue.
Researchers place biomaterials and tissue-engineered constructs in a defined cardiac model to examine their effects on myocardial mechanics and heart function. The organ’s experimental accessibility supports direct assessment of how an intervention behaves in a living cardiac setting. These studies can help determine whether a design merits further development for repair-oriented engineering applications.
Murine-heart studies provide a setting for evaluating whether a drug-delivery system produces measurable cardiac responses. Researchers can relate the intervention to changes in contraction, myocardial mechanics, or overall function rather than considering delivery in isolation. This makes the model useful for examining how an engineered delivery approach may influence cardiac performance and repair strategies.
Imaging methods can document cardiac structure and functional responses, while computational models help represent or analyze the mechanics and circulation associated with those observations. Used together, they provide complementary evidence for evaluating devices, biomaterials, and constructs. In engineering research, this combination supports comparison between predicted behavior and measured heart performance.