Physical Crosslinking
Physical Crosslinking - Related Videos
03:47
Crosslinking Neuronal Surface Proteins in the Mouse Brain using a Chemical Crosslinking Assay
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2025
This video demonstrates the crosslinking of neuronal surface proteins using Bis-sulfosuccinimidyl suberate or BS3. Isolated and chilled mouse brain is sectioned coronally. The desired region is extracted from the sections, minced, and treated with BS3. BS3 crosslinks surface proteins like GABA receptors, leaving internal proteins unaltered. The reaction is quenched with glycine before processing the samples for further analysis.
11:15
Nanosponge Tunability in Size and Crosslinking Density
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Cited by 1 •
2017
This article describes a process for tuning the size and crosslinking density of covalently crosslinked nanoparticles from linear polyesters containing pendant functionality. By tailoring synthesis parameters (polymer molecular weight, pendant functionality incorporation, and crosslinker equivalents), a desired nanoparticle size and crosslinking density can be achieved for drug delivery applications.
09:06
Preparation of DNA-crosslinked Polyacrylamide Hydrogels
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Cited by 5 •
2014
Our laboratory has developed DNA-crosslinked polyacrylamide hydrogels, a dynamic hydrogel system, to better understand the effects of modulating tissue stiffness on cell function. Here, we provide schematics, descriptions, and protocols to prepare these hydrogels.
10:12
Quantitative Detection of DNA-Protein Crosslinks and Their Post-Translational Modifications
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Cited by 3 •
2023
The present protocol highlights a modified method to detect and quantify DNA-protein crosslinks (DPCs) and their post-translational modifications (PTMs), including ubiquitylation, SUMOylation, and ADP-ribosylation induced by topoisomerase inhibitors and by formaldehyde, thereby allowing the study of the formation and repair of DPCs and their PTMs.
05:21
General Approach to the Physical Exam
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2023
Source: Jaideep S. Talwalkar, MD, Internal Medicine and Pediatrics, Yale School of Medicine, New Haven, CT The examination of the body is fundamental to the practice of medicine. Since the Roman Empire, physicians have described the connection between alterations in function of specific parts of the body and specific disease states and have sought to further scientific understanding to improve bedside diagnosis. However, in this modern age of increasing technology within medical diagnostics, it...
FAQs
The network is governed by the type and distribution of reversible associations between polymer chains. Hydrogen bonds, ionic attractions, hydrophobic associations, crystallites, and structures produced by repeated freeze-thaw cycles can each connect chains in different ways. Because polymer composition determines which associations are available, it strongly influences the resulting gel architecture and behavior.
Reversible associations allow the network to reorganize rather than remaining permanently fixed. This behavior can support self-healing after structural disruption, stimuli-responsive changes when environmental conditions shift, and tunable degradation. Compared with a permanently covalent network, the physical network therefore offers dynamic behavior that can be useful when a biomaterial must adapt during biological use.
Environmental conditions can change how polymer chains associate and how stable the resulting network remains. Since gel structure depends on both polymer composition and surroundings, altering those conditions may influence hydrogen bonding, ionic interactions, hydrophobic associations, crystallite formation, or freeze-thaw-driven organization. This sensitivity enables researchers to tune gel properties for specific bioengineering requirements.
Design begins by selecting a polymer composition that can form the desired reversible associations, then using an appropriate network-forming condition, such as ionic interaction, hydrogen bonding, hydrophobic association, crystallite formation, or repeated freeze-thaw treatment. Researchers adjust the composition and environment to obtain a gel with suitable structure, responsiveness, degradation behavior, and compatibility with biological components.
This approach is valuable when processing conditions must remain mild enough to help preserve sensitive cells, proteins, or other biological components. Its reversible network can also support injectable materials, where the gel must provide useful structure while retaining dynamic behavior. These features make the method relevant to several bioengineering designs rather than only to permanent structural scaffolds.
Physically crosslinked networks can be incorporated into injectable hydrogels, tissue-engineering scaffolds, wound dressings, and controlled drug-delivery systems. Their reversible associations allow designers to adjust structural behavior and degradation, while the mild formation conditions can help protect biological cargo or cells. The same underlying strategy can therefore serve delivery, repair, and tissue-support functions.