Dynamic Compliance Analysis

Dynamic compliance analysis is a method for quantifying how a material, tissue, or biological system deforms in response to forces that change over time. It typically applies controlled, time-dependent stress or pressure and measures the resulting strain or volume change, revealing compliance, viscoelastic behavior, and the phase relationship between applied force and deformation. In bioengineering, these measurements help characterize soft biomaterials, engineered tissues, and biological structures under conditions that better reflect physiological loading than static tests. The resulting mechanical profiles support material selection, device design, tissue modeling, and evaluation of how engineered systems may perform during repeated or dynamic use.

Dynamic Compliance Analysis - Related Videos

Research

JoVE Journal - Bioengineering

Quantifying Inferior Vena Cava Compliance and Distensibility in an In Vivo Ovine Model Using 3D Angiography

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Cited by 2 •

2024

This protocol allows for the in vivo quantification of venous compliance and distensibility using catheterization and 3D angiography as a survival procedure allowing for a variety of potential applications.

Correlative Microscopy for 3D Structural Analysis of Dynamic Interactions

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Cited by 15 •

2013

We describe a correlative microscopy method that combines high-speed 3D live-cell fluorescent light microscopy and high-resolution cryo-electron tomography. We demonstrate the capability of the correlative method by imaging dynamic, small HIV-1 particles interacting with host HeLa cells.

Comprehensive Analysis of Transcription Dynamics from Brain Samples Following Behavioral Experience

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Cited by 6 •

2014

This manuscript describes a protocol that applies comprehensive profiling for analysis of transcriptional programs induced in specific brain nuclei of rodents following behavioral paradigms. Herein, this approach is illustrated in the context of profiling genes induced in the nucleus accumbens (NAc) of mice following acute cocaine exposure, utilizing microfluidic qPCR arrays.

Research

JoVE Journal - Bioengineering
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Quantifying Cytoskeleton Dynamics Using Differential Dynamic Microscopy

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Cited by 13 •

2022

Differential dynamic microscopy (DDM) combines features of dynamic light scattering and microscopy. Here, the process of using DDM to characterize reconstituted cytoskeleton networks by quantifying the subdiffusive and caged dynamics of particles in vimentin networks and the ballistic motion of active myosin-driven actin-microtubule composites is presented.

High-resolution Imaging and Analysis of Individual Astral Microtubule Dynamics in Budding Yeast

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Cited by 26 •

2017

Budding yeast is an advantageous model for studying microtubule dynamics in vivo due to its powerful genetics and the simplicity of its microtubule cytoskeleton. The following protocol describes how to transform and culture yeast cells, acquire confocal microscopy images, and quantitatively analyze microtubule dynamics in living yeast cells.

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