6:10Non-Viral Engineering of Primary Human T Cells via Homology-Mediated End-Joining Targeted Integration of Large DNA Templates0 views•2025•Biology•Topical CollectionMatthew J. Johnson1,2,3, Anthony P. DeFeo1,2,3, Nicholas J. Slipek1,2,3, Timothy D. Folsom1,2,3, Tom Henley4, Modassir S. Choudhry4, Beau R. Webber1,2,3,5, Branden S. Moriarity1,2,3,5Matthew J. Johnson1,2,3Anthony P. DeFeo1,2,3Nicholas J. Slipek1,2,3Timothy D. Folsom1,2,3Tom Henley4Modassir S. Choudhry4Beau R. Webber1,2,3,5Branden S. Moriarity1,2,3,51Department of Pediatrics, University of Minnesota, 2Masonic Cancer Center, University of Minnesota, 3Center for Genome Engineering, University of Minnesota, 4Intima Bioscience, Inc., 5Stem Cell Institute, University of MinnesotaDepartment of Pediatrics, University of MinnesotaMasonic Cancer Center, University of MinnesotaCenter for Genome Engineering, University of MinnesotaIntima Bioscience, Inc.Stem Cell Institute, University of Minnesota