<p class="jove_content">This protocol describes a reproducible, Langendorff-free method for isolating viable adult mouse ventricular cardiomyocytes from a doxorubicin-induced cardiotoxicity model. It employs direct left-ventricular antegrade perfusion followed by controlled enzymatic digestion to yield calcium-tolerant, rod-shaped myocytes suitable for structural, molecular, and functional analyses. </p>

isolating-ventricular-cardiomyocytes-from-mouse-model-doxorubicin

Isolating Ventricular Cardiomyocytes from a Mouse Model of Doxorubicin Cardiotoxicity

Doxorubicin (DOX) is an effective chemotherapeutic drug whose clinical application is limited by progressive, dose-dependent cardiotoxicity that ...

<p class="jove_content">This protocol details the application of implantable telemetry for continuous and reliable arterial pressure monitoring in conscious rodents, covering surgical implantation, postoperative care, data acquisition, and comparison with the tail-cuff method.</p>

continuous-arterial-pressure-monitoring-conscious-rodents-using

Continuous Arterial Pressure Monitoring in Conscious Rodents Using Implantable Telemetry and Comparison with the Tail-Cuff Method

Radio telemetry enables reliable, continuous arterial blood pressure (BP) monitoring in conscious rodents under physiological conditions, while minimizing ...

Institution - East China Normal University Health Science Center

Articles Published in JoVE