<p class="jove_content">The preparation of stable isotope-labeled vitamin A, necessary for the retinol isotope dilution technique, needs to conform to food safety standards. The protocol shows dosage preparation in vegetable oil at concentrations that will be suitable for the chosen delivery system and the applied analytical platform. </p>

formulation-vitamin-tracer-doses-for-administration-to-human-subjects

Formulation of Vitamin A Tracer Doses for Administration to Human Subjects using Retinol Isotope Dilution Technique

The retinol isotope dilution (RID) technique relies on the administration of isotopically labeled vitamin A tracers to human subjects. Here, we present a ...

<p class="jove_content">Here, we present a protocol to simultaneously quantify both isotopically labeled and native retinoids in human plasma, using LC-MS/MS, with detection limits of 6 fmol on-column. The method incorporates a simple sample extraction procedure and a short analytical run time, enabling a high throughput of samples.</p>

analysis-of-vitamin-a-stable-isotope-tracers-using-lc-msms

Analysis of Vitamin A Stable Isotope Tracers using LC-MS/MS

The retinol isotope dilution (RID) method estimates the total body stores (TBS) of vitamin A in humans through stable isotope dilution with the body&#39;s ...

Institution - Fera Science Ltd

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