This protocol outlines methods to culture, manipulate, and analyze murine CAFs, including in vitro/in vivo functional assays, transcriptomics, and computational analysis. Moreover, it describes a web-based platform developed to analyze the publicly available transcriptomes of laser capture micro-dissected stromal and epithelial components from a collection of human breast cancer tumors.

cancer-associated-fibroblasts-from-mouse-mammary-tumors-as-tools-for

Cancer-Associated Fibroblasts from Mouse Mammary Tumors as Tools for Molecular and Computational Studies

A compelling body of research has confirmed the critical role of the tumor microenvironment (TME) in the growth and aggressiveness of breast cancer (BC). ...

Presented here is a protocol to perform genetic manipulation in the embryonic ferret brain using in utero electroporation. This method allows for targeting of neural progenitor cells in the neocortex in vivo.

in-vivo-targeting-neural-progenitor-cells-ferret-neocortex-utero

In Vivo Targeting of Neural Progenitor Cells in Ferret Neocortex by In Utero Electroporation

Manipulation of gene expression in vivo during embryonic development is the method of choice when analyzing the role of individual genes during mammalian ...

Institution - Human Technopole

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