Antibiotic Conjugation
Antibiotic Conjugation - Related Videos
13:39
Antibiotic Susceptibility Testing: Epsilometer Tests to Determine MIC Values of Two Antibiotics and Evaluate Antibiotic Synergy
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2023
Source: Anna Bläckberg1, Rolf Lood1 1 Department of Clinical Sciences Lund, Division of Infection Medicine, Biomedical Center, Lund University, 221 00 Lund Sweden Knowledge of the interactions between antibiotics and bacteria is important in understanding how microbes evolve antibiotic resistance. In 1928, Alexander Fleming discovered penicillin, an antibiotic that exerts its antibacterial function by interfering with cell wall regeneration (1). Other antibiotics with diverse mechanisms of...
02:50
Conjugated Proteins
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2020
Simple proteins and protein complexes contain only amino acids. In contrast, many other proteins, called conjugated proteins, covalently bond with non-protein moieties. Nucleoproteins are protein complexes that contain nucleic acids, categorized as deoxyribonucleoproteins (DNPs) or ribonucleoproteins (RNPs) respectively. The nucleosome is a typical example of a DNP where nuclear DNA is associated with histone proteins. The major antigen for the Covid-19 virus SARS-CoV is an RNP that is critical...
10:49
Antibiotic Dereplication Using the Antibiotic Resistance Platform
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Cited by 2 •
2019
We describe a platform that utilizes a library of isogenic antibiotic resistant Escherichia coli for the dereplication of antibiotics. The identity of an antibiotic produced by bacteria or fungi can be deduced by the growth of E. coli expressing its respective resistance gene. This platform is economically effective and time-efficient.
08:58
Isolation and Identification of Waterborne Antibiotic-Resistant Bacteria and Molecular Characterization of their Antibiotic Resistance Genes
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Cited by 5 •
2023
Here, we present a detailed protocol for the isolation and identification of antibiotic-resistant bacteria from water and the molecular characterization of their antibiotic resistance genes (ARGs). The use of culture-based and non-culture-based (metagenomic analysis) techniques provides complete information about the total bacterial diversity and the total pool of different ARGs present in freshwaters from Mumbai, India.
14:04
Biosensor for Detection of Antibiotic Resistant Staphylococcus Bacteria
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Cited by 17 •
2013
Lytic phage biosensors and antibody beads are able to discriminate between methicillin resistant (MRSA) and sensitive staphylococcus bacteria. The phages were immobilized by a Langmuir-Blodgett method onto a surface of a quartz crystal microbalance sensor and worked as broad range staphylococcus probes. Antibody beads recognize MRSA.
FAQs
The linker determines whether the attached antibiotic remains joined to its partner during delivery or is released after encountering specified chemical or biological conditions. A stable linker can preserve the conjugate until it reaches its intended site, whereas a cleavable linker supports release of the active drug there. This choice therefore connects molecular design with delivery performance and antibacterial action.
Carriers, targeting ligands, and imaging agents contribute different functions to the same conjugate design. A carrier can help modify solubility or stability, while a targeting ligand can influence where the antibiotic reaches bacterial cells. An imaging agent can add a diagnostic role, allowing therapeutic and diagnostic functions to be combined within one platform.
Compared with an unmodified antibiotic, attaching another molecule can change how the drug reaches and acts on bacterial cells. This altered delivery may support more localized exposure, reduce off-target exposure, or improve solubility and stability. The conjugate therefore serves as an adaptable delivery format when the engineering goal extends beyond the antibiotic's intrinsic activity.
A conceptual design workflow starts with choosing the antibiotic and the second molecule according to the desired delivery, targeting, stability, solubility, or imaging function. Engineers then define a covalent linker that either persists during delivery or can be cleaved under selected chemical or biological conditions. This sequence connects molecular composition to the intended site and therapeutic outcome.
These settings are relevant because the approach can be adapted to influence where an antibiotic reaches bacterial cells and how the active drug is released. A conjugate may support targeted delivery at an intended site, limit off-target exposure, or provide a platform for addressing drug-resistant infections. These uses represent potential engineering applications rather than guaranteed clinical outcomes.
An imaging agent can be attached alongside the antibiotic through the conjugate design, creating a platform with both therapeutic and diagnostic functions. The antibiotic component supports antimicrobial action, while the imaging component adds diagnostic capability. In bioengineering, this combination provides an adaptable molecular platform for integrating treatment and diagnostic purposes within the same conjugate.