Laser-induced Fluorescence
Laser-induced Fluorescence - Related Videos
06:20
Flapping Soft Fin Deformation Modeling using Planar Laser-Induced Fluorescence Imaging
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Cited by 1 •
2022
The present protocol involves the measurement and characterization of 3D shape deformation in underwater flapping fins built with polydimethylsiloxane (PDMS) materials. Accurate reconstruction of these deformations is essential for understanding the propulsive performance of compliant flapping fins.
08:07
Laser-induced Forward Transfer of Ag Nanopaste
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Cited by 15 •
2016
We demonstrate the use of the Laser-induced forward transfer technique (LIFT) for the printing of high-viscosity Ag paste. This technique offers a simple, low temperature, robust process for non-lithographically printing microscale 2D and 3D structures.
05:44
Research Application of Laser-Induced Shock Wave for Studying Blast-Induced Cochlear Injury
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2024
Here, we describe experimental protocols for creating an animal model of blast-induced cochlear injury using laser-induced shock wave (LISW). Exposure of the temporal bone to LISW allows the reproduction of blast-induced cochlear pathophysiology. This animal model could be a platform for elucidating cochlear pathology and exploring potential treatments for blast injuries.
07:08
A Mouse Model for Laser-induced Choroidal Neovascularization
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Cited by 87 •
2015
Here, we present the mouse laser-induced choroidal neovascularization (CNV) protocol, an experimental model that re-creates the vascular hallmarks of neovascular age-related macular degeneration (AMD). Once mastered, it can reliably and effectively induce CNV as a model system to test various experimental measures.
02:30
Performing Laser-Induced Brain Injury in a Rat Model
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2025
This video demonstrates a procedure to generate precise, controlled brain injury in the rat motor cortex using a laser.
FAQs
Matching the laser wavelength to an absorption transition allows fluorescent species to absorb photons and briefly occupy an excited electronic state. As they return toward the ground state, they emit light at a longer wavelength than the excitation light. This separation between excitation and emission supports optical detection of the fluorescent signal from the measured region.
The measured intensity carries information about both the amount of fluorescent species and the environment in which they are observed. Higher or lower signal should therefore be interpreted in relation to concentration and local conditions rather than as a concentration-only readout. In bioengineering experiments, this makes the emitted signal useful for assessing biochemical or cellular changes within a defined optical measurement region.
A focused laser beam confines excitation to a selected region, which helps preserve spatial detail in measurements of cells, tissues, or microfluidic channels. The technique's temporal resolution also allows signal changes to be followed as biochemical or cellular processes evolve. These properties explain its use for localized imaging and monitoring of dynamic biological processes.
A basic measurement starts by choosing a laser wavelength that matches an absorption transition in the fluorescent species. The beam is focused on the region of interest, and the emitted, longer-wavelength light is detected. Researchers can interpret the resulting intensity in relation to species concentration and local conditions while maintaining noncontact optical access to the sample.
In microfluidic devices, the technique enables sensitive, noncontact analysis of cells, proteins, metabolites, and fluorescent labels. Its localized optical measurement can also support flow measurements and monitoring of biochemical or cellular processes within small device regions. High spatial and temporal resolution helps connect fluorescence signals with changes occurring as biological material moves or evolves through the system.
Within biological tissues, Laser-induced Fluorescence supports noncontact analysis of fluorescent species and labeled biological targets. The emitted signal can reflect concentration and local conditions, while the method's spatial and temporal resolution enables imaging and process monitoring. These capabilities make it relevant to bioengineering applications involving diagnostics and the observation of biochemical or cellular behavior.