Organoid Units
Organoid Units - Related Videos
05:38
Generation of Apical-Out Intestinal Organoids and Assessment of Organoid Proliferation Rate Using EdU-Labeling
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Cited by 1 •
2025
Here, we describe a protocol for generating apical-out intestinal organoids from standard matrix-embedded organoid cultures. It also outlines the subsequent incorporation of EdU into actively proliferating cells and the semiautomatic quantification of EdU-positive cells.
10:56
Combining Human Organoids and Organ-on-a-Chip Technology to Model Intestinal Region-Specific Functionality
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Cited by 9 •
2022
Biopsy-derived intestinal organoids and organ-on-a-chips technologies are combined into a microphysiological platform to recapitulate region-specific intestinal functionality.
03:38
Measurement: Standard Units
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2020
Every measurement provides three kinds of information: the size or magnitude of the measurement (a number), a standard of comparison for the measurement (a unit), and an indication of the uncertainty of the measurement. While the number and unit are explicitly represented when a quantity is written, the uncertainty is an aspect of the errors in the measurement results. The number in the measurement can be represented in different ways, including decimal form and scientific notation, which is...
03:02
Measurement: Derived Units
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2020
The International System of Units or SI system, by international agreement, has fixed measurement units for seven fundamental properties: length, mass, time, temperature, electric current, amount of substance, and luminosity. These are called the SI base units. Units of measurement derived from the mathematical combination of SI base units are called SI-derived units. For example, the ratio of the SI unit for distance (meter; m) and the SI unit for time (second; s) gives the SI-derived unit...
05:11
Organoid Electroporation: A Method to Transfect Plasmid DNA into Gastrointestinal Organoids
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2023
This video describes the electroporation protocol for transfecting large plasmids into three-dimensional gastrointestinal organoids to prove its universal functionality in different entities, namely pancreatic ductal adenocarcinoma (PDAC), colorectal cancer (CRC), cholangiocarcinoma (CCC), and gastric cancer (GC) organoids.
FAQs
Stem and progenitor cells provide the starting populations from which organoid units acquire specialized cell states and organized architecture. Their behavior depends on signals supplied by the culture environment, rather than on cell identity alone. This makes the system useful for examining how developmental cues and engineered surroundings jointly influence tissue-like structure and function.
Defined biochemical and physical conditions regulate whether cells remain progenitor-like, differentiate, and organize in space. Changing these inputs can therefore alter the resulting assembly’s composition, architecture, and functional characteristics. In bioengineering studies, controlling the conditions helps investigators attribute observed changes to particular environmental cues instead of treating the organoid unit as a fixed biological output.
An extracellular-matrix-based scaffold provides more than physical support. It helps cells maintain polarity, meaning an organized orientation within the assembly, while also supporting differentiation and spatial arrangement. Because scaffold properties are part of the culture environment, modifying the matrix offers a way to study how surrounding materials influence organoid organization and to evaluate engineered culture systems.
Researchers can vary the biochemical environment, physical conditions, and extracellular-matrix-based scaffold, then examine how those changes affect organization and function. This creates a controllable platform for comparing biomaterials or culture configurations under defined conditions. The resulting differences help identify design features that produce more physiologically relevant in vitro models.
A general workflow begins by maintaining stem or progenitor cells under selected biochemical and physical conditions, often with an extracellular-matrix-based scaffold. Researchers then assess how cells differentiate, establish polarity, and organize spatially. Finally, they relate the observed structure and function to the culture design or experimental question, such as development, disease, or drug response.
Researchers can use organoid units when a study requires three-dimensional organization alongside cell differentiation and tissue-level features. They support investigations of developmental processes, disease-associated biology, and drug responses while also enabling evaluation of biomaterials and culture systems. This combination connects biological questions with engineering choices that shape structure and function in vitro.
In regenerative medicine, organoid units provide an experimental intermediate for studying how cells and engineered environments can be organized toward tissue-relevant outcomes. Their tunable composition and architecture support evaluation of culture and material strategies within tissue-engineering research. This helps connect controllable in vitro design with broader efforts to develop more physiologically relevant models and regenerative approaches.