Photothermal Ablation
Photothermal Ablation - Related Videos
09:51
One Minute, Sub-One-Watt Photothermal Tumor Ablation Using Porphysomes, Intrinsic Multifunctional Nanovesicles
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Cited by 15 •
2013
We developed novel intrinsic multifunctional nanovesicles called porphysomes, which have structure-dependent fluorescence self-quenching and unique photothermal properties, thus functioning as potent photothermal therapy agents. We formulated porphysomes using high pressure extrusion and investigated their photothermal therapy efficacy in a xenograft tumor model.
04:07
Optical Photothermal Infrared-Fluorescence In Situ Hybridization (OPTIR-FISH)
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Cited by 1 •
2024
Here, we present a protocol using optical photothermal infrared-fluorescence in situ hybridization (OPTIR-FISH), also known as mid-infrared photothermal-FISH (MIP-FISH), to identify individual cells and understand their metabolism. This methodology can be applied broadly for diverse applications, including mapping cellular metabolism with single-cell resolution.
06:42
Plasmonic Photothermal Cancer Therapy: Nanoparticle-embedded Tumor-tissue-mimicking Phantoms for Visualizing Photothermal Temperature Distribution
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Cited by 1 •
2025
This article presents the protocol for preparing tumor-tissue phantoms that replicate optical properties for plasmonic photothermal therapy. It details phantom preparation, photothermal evaluations, and validation of the developed numerical model based on photothermal temperature measurements for assessing therapeutic parameters, offering an ethical, cost-effective alternative to in vivo studies for preliminary testing.
10:16
Electroactive Polymer Nanoparticles Exhibiting Photothermal Properties
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Cited by 5 •
2016
A protocol is presented for the synthesis and preparation of nanoparticles consisting of electroactive polymers.
10:02
Submillisecond Conformational Changes in Proteins Resolved by Photothermal Beam Deflection
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Cited by 1 •
2014
Here we report an application of the photothermal beam deflection technique in combination with a caged calcium compound, DM-nitrophen, to monitor microsecond and millisecond dynamics and energetics of structural changes associated with the calcium association to a neuronal calcium sensor, Downstream Regulatory Element Antagonist Modulator.
FAQs
Absorbing materials serve as the energy-conversion point in photothermal ablation. After illumination, they convert optical energy into heat near the selected target rather than distributing the treatment uniformly across the exposed region. This localized conversion helps researchers concentrate thermal damage in intended cells or tissue and supports efforts to limit unnecessary tissue injury.
Particle design, light wavelength, exposure time, and heat distribution all influence the balance between treatment effectiveness and safety. These variables determine how efficiently the target absorbs light, how long heat persists, and how widely it spreads. Researchers adjust them to improve control over local tissue damage while maintaining temperatures sufficient for the intended cellular response.
The spatial distribution of heat determines whether thermal effects remain concentrated in the selected target or extend into adjacent tissue. Excessively broad heating can reduce treatment precision, whereas insufficient local heating may fail to produce the desired cellular damage. For this reason, bioengineering studies examine heat distribution alongside exposure conditions when evaluating safety and effectiveness.
A typical workflow begins by selecting a target and an absorbing material, such as a photothermal agent or nanoparticle. A laser or another light source then illuminates the material for a defined exposure time. Researchers evaluate how the resulting heat affects the targeted cells or tissue and use those observations to refine particle design and illumination conditions.
Researchers investigate this approach when they need localized treatment, including tumor therapy or minimally invasive tissue ablation. Its value comes from combining an absorbing material with controlled illumination so that treatment can be directed toward a selected region. Studies focus on achieving useful cellular destruction while improving control over the extent of surrounding tissue damage.
Bioengineering contributes by examining both the treatment system and its controllable variables, including photothermal particle design, light wavelength, exposure time, and heat distribution. These investigations connect material properties and illumination conditions with cellular outcomes. The resulting knowledge supports development of more precise therapies and helps researchers improve the balance between effective ablation and tissue safety.