Phase Correlation Registration

Phase correlation registration is an image-alignment method that estimates the translation needed to superimpose two images, making it valuable for correcting motion and comparing biological structures. It uses the Fourier shift theorem: after transforming both images into the frequency domain, their normalized cross-power spectrum isolates phase differences caused by displacement, and an inverse Fourier transform produces a sharp peak at the relative shift. In bioengineering, the method supports microscopy, medical imaging, and time-lapse analysis by aligning frames despite changes in position or acquisition conditions. Its computational efficiency and reduced sensitivity to intensity variations help quantify structural changes, track specimens, and improve downstream image analysis.

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JoVE Business - Finance

Shelf Registration

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2026

Shelf registration is an essential tool for companies to raise capital flexibly and efficiently. It is a provision by the Securities and Exchange Commission (SEC) that enables companies to file a single registration for securities they intend to issue over time rather than all at once. This process offers multiple advantages.Flexibility and Convenience: Shelf registration allows companies to issue securities in phases per market conditions and funding needs rather than raising all capital at...

Correlations

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2020

Correlation means that there is a relationship between two or more variables (such as ice cream consumption and crime), but this relationship does not necessarily imply cause and effect. When two variables are correlated, it simply means that as one variable changes, so does the other. We can measure correlation by calculating a statistic known as a correlation coefficient. A correlation coefficient is a number from -1 to +1 that indicates the strength and direction of the relationship between...

Correlation and Causation

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2026

Correlation and CausationStatistical tests can calculate whether there is a relationship, or correlation, between independent and dependent variables. A relationship between variables shows correlation, but it does not show cause-and-effect. A direct cause-and-effect relationship requires additional controlled experiments. If no consistent relationship exists between the variables, then there is no correlation.Correlation versus CausationIf the dependent variable increases or decreases when the...

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JoVE Journal - Biology

Epon Post Embedding Correlative Light and Electron Microscopy

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Cited by 1 •

2024

We present a detailed protocol for Epon post-embedding correlative light and electron microscopy using a fluorescent protein called mScarlet. This method can maintain the fluorescence and the ultrastructure simultaneously. This technique is amenable to a wide variety of biological applications.

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JoVE Journal - Medicine
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Three-Dimensional Phase Resolved Functional Lung Magnetic Resonance Imaging

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Cited by 4 •

2024

Three-dimensional (3D) phase-resolved functional lung (PREFUL) is a functional magnetic resonance imaging (MRI) technique that allows for quantification of regional ventilation of the whole human lung volume, using tidal breathing and contrast-agent free acquisition for 8 min. Here, we present an MR protocol to collect and analyze 3D PREFUL imaging data.

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