Cellular Reprogramming
Cellular Reprogramming - Related Videos
09:14
Evaluation of Injury-induced Senescence and In Vivo Reprogramming in the Skeletal Muscle
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Cited by 15 •
2017
Here we present a detailed protocol to detect both senescent and pluripotent stem cells in the skeletal muscle upon injury while inducing in vivo reprogramming. This method is suitable for evaluating the role of cellular senescence during tissue regeneration and reprogramming in vivo.
07:08
Reprogramming Pancreatic Ductal Adenocarcinoma to Pluripotency
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2024
The present protocol describes the reprogramming of Pancreatic Ductal Adenocarcinoma (PDAC) and normal pancreatic ductal epithelial cells into induced pluripotent stem cells (iPSCs). We provide an optimized and detailed, step-by-step procedure, from preparing lentivirus to establishing stable iPSC lines.
09:38
Direct Reprogramming of Mouse Fibroblasts into Melanocytes
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Cited by 2 •
2021
Here, we describe an optimized direct reprogramming system for melanocytes and a high-efficiency, concentrated virus packaging system that ensures smooth direct reprogramming.
02:12
Direct Neuronal Reprogramming of Mouse Astrocytes into Neurons
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2025
This video showcases the direct reprogramming of astrocytes expressing transforming factors into neurons. Transformation factors trigger a cascade of molecular events facilitating astrocyte-to-neuron conversion. The process is further supported by supplying specific agents and supplements through a neuronal differentiation medium.
09:34
Reprogramming Primary Amniotic Fluid and Membrane Cells to Pluripotency in Xeno-free Conditions
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Cited by 3 •
2017
This protocol describes the reprogramming of primary amniotic fluid and membrane mesenchymal stem cells into induced pluripotent stem cells using a non-integrating episomal approach in fully chemically defined conditions. Procedures of extraction, culture, reprogramming, and characterization of the resulting induced pluripotent stem cells by stringent methods are detailed.
FAQs
Defined transcription factors drive reprogramming by shifting gene regulation in two directions: they activate genes associated with pluripotency while suppressing programs that maintain the mature cell’s original identity. This opposing control is important because changing cell behavior requires more than turning on a new set of genes; the former differentiated program must also be reduced.
The epigenetic state matters because reprogramming resets a layer of cellular information alongside gene expression. Scientists can therefore use the process to examine epigenetic memory and ask whether features of the original differentiated state remain relevant after identity changes. This makes reprogrammed cells useful for studying how prior cell history influences later cellular behavior experimentally.
A key biological outcome is the acquisition of pluripotency-associated potential rather than merely a temporary change in appearance. Once generated, induced pluripotent stem cells can produce many specialized cell types. That capacity lets investigators connect control of cell identity with development and test whether a reprogrammed population can serve as a starting point for studying differentiated tissues.
A typical workflow starts with mature differentiated cells, exposes them to defined transcription factors, and monitors conversion toward induced pluripotent stem cells. The resulting cells are then used as a source for generating specialized cell types. This sequence links the molecular intervention, the identity change, and the downstream biological models used in experiments.
Cellular reprogramming supports several lines of investigation: development, disease modeling, drug testing, regenerative medicine, and cell-based therapies. It also gives biology researchers a way to examine cell fate control and the relationship between gene expression, epigenetic state, and cellular identity. Together, these uses connect basic studies of fate with practical experimental goals.
The process gives researchers a way to connect altered cell identity with the regulation of gene expression and epigenetic state. By examining cells after identity has been changed, studies can ask how these regulatory features relate to disease mechanisms. This context complements disease modeling and supports evaluation of potential cell-based therapeutic strategies.