Anterior Segment Imaging

Anterior segment imaging is the noninvasive visualization and measurement of the eye’s front structures, including the cornea, iris, anterior chamber, and lens, supporting diagnosis, treatment planning, and device development. Depending on the modality, it uses reflected or transmitted light, optical sectioning, or interferometric measurements to produce two- or three-dimensional images and quantify tissue shape, thickness, and transparency. In engineering, these systems combine optical design, sensors, image processing, and computational analysis to improve resolution, speed, and reliability. Applications include evaluating corneal disease, planning refractive and cataract procedures, assessing intraocular lenses, and guiding the development of ophthalmic instruments.

Anterior Segment Imaging - Related Videos

Research

JoVE EoE - Large Animal Models

Anterior Eye Segment Dissection: A Method to Isolate Cornea Bearing Anterior Segment from Porcine Eye

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2025

In this video, we demonstrate the dissection of the cornea-bearing anterior segment of the porcine eye. The isolated segment can be used for organ culturing to study the pathophysiology of eye disorders.

Anterior Eye Segment Culturing: A Method to Culture Cornea Bearing Anterior Segment of Porcine Eye

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2025

In this video, we demonstrate the culturing method of a cornea-bearing anterior eye segment from a porcine eye. This culture system serves as an experimental model to study eye injuries.

Anterior Segment Organ Culture Platform for Tracking Open Globe Injuries and Therapeutic Performance

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Cited by 1 •

2021

Open globe eye injuries may go untreated for multiple days in rural or military-relevant scenarios, resulting in blindness. Therapeutics are needed to minimize loss of vision. Here, we detail an organ culture open globe injury model. With this model, potential therapeutics for stabilizing these injuries can be properly evaluated.

Research

JoVE Journal - Medicine

Urethroplasty with Pedicled Tunica Vaginalis for the Treatment of Long-segment Anterior Urethral Stricture Caused by Lichen Sclerosus of Glans Penis

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Cited by 1 •

2024

This article introduces a surgical method using a pedicled tunica vaginalis to treat long-segment urethral stricture resulting from lichen sclerosus. The tunica vaginalis is an excellent substitute for the urethra, leading to quick postoperative recovery for patients.

Research

JoVE Journal - Biology
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In Vivo Imaging of Liver Spheroids Engrafted in the Anterior Chamber of the Mouse Eye

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2024

Here, we describe a platform that allows noninvasive in vivo imaging of liver spheroids engrafted in the anterior chamber of the mouse eye. The workflow spans from generating spheroids from primary liver cells to transplantation into the mouse eye and in vivo imaging at cellular resolution by confocal microscopy.

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