Electrode Disc Punching
Electrode Disc Punching - Related Videos
01:32
Pinching-off of Coated Vesicles
0 Views •
2023
Vesicle budding is orchestrated by distinct cytosolic proteins such as adaptor proteins, coat proteins, and GTPases. To initiate vesicle budding, membrane-bending proteins containing crescent-shaped BAR domains bind to the lipid heads in the bilayer and distort the membrane to form a protein-coated vesicle bud. Adaptors proteins such as AP2 for clathrin-coated vesicles can nucleate on the deformed membrane. Finally, coat proteins such as clathrin or COPI and COPII assemble into a coat forming...
08:14
Induction and Diagnosis of Tumors in Drosophila Imaginal Disc Epithelia
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Cited by 9 •
2017
Mosaic clone analysis in Drosophila imaginal disc epithelia is a powerful model system to study the genetic and cellular mechanisms of tumorigenesis. Here we describe a protocol to induce tumors in Drosophila wing imaginal discs using the GAL4-UAS system, and introduce a diagnosis method to classify the tumor phenotypes.
03:12
Drosophila Larva Imaginal Disc Dissection: A Method to Observe Developing Epithelia
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2023
When staining Drosophila imaginal discs, often a rough dissection is performed where the discs are exposed but remain attached to the body. Once stained, the discs of interest are isolated and mounted. The example protocol demonstrates a procedure for wing imaginal disc dissection and mounting.
07:48
Dissection and Mounting of Drosophila Pupal Eye Discs
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Cited by 6 •
2014
The goal of this technique is to enable researchers to perform dissection, immunostaining and mounting of pupal eye discs from Drosophila melanogaster of any age.
06:08
Long-term Live Imaging of Drosophila Eye Disc
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Cited by 6 •
2017
This protocol describes a detailed method for the long-term ex vivo culture and live imaging of a Drosophila imaginal disc. It demonstrates photoreceptor differentiation and ommatidial rotation within the 10 h period of live imaging of the eye disc. The protocol is simple and does not require expensive setup.
FAQs
A punch applies controlled force against a die, causing the coated material to shear along the tool’s cutting boundary. This controlled separation determines the disc’s circular geometry while limiting unwanted deformation, edge damage, and loss of the electrode coating. Consistent shearing is important because each sample can then represent a comparable electrode area during electrochemical testing.
Consistent diameter is the main dimensional control, but the cut edge also affects sample quality. Excessive deformation can alter the disc shape, while edge damage or coating loss can reduce the material actually available for testing. Controlling the applied force helps preserve comparable samples, making differences among electrode formulations more likely to reflect the materials rather than inconsistent preparation.
A consistent circular area makes area calculations comparable across samples. That consistency supports more reliable current-density measurements because the measured current can be related to a known electrode area. It also helps compare mass loading and electrochemical behavior among formulations, which is essential when engineering studies seek reproducible device results.
The workflow begins with a coated metal foil or composite sheet, which is positioned for cutting with a punch-and-die tool. Controlled force then shears out circular samples. The resulting discs can be used for electrochemical testing or device assembly, where their consistent dimensions support standardized comparisons. This workflow links physical sample preparation directly to later area and loading calculations.
Standardized discs provide a consistent basis for determining electrode area and comparing mass loading. Those quantities are needed when interpreting current-density measurements, since the applied or measured current is evaluated relative to electrode area. By reducing preparation variability, punching helps researchers compare formulations and assess electrochemical results with greater confidence.
Its applications extend across batteries, supercapacitors, fuel cells, and related electrochemical systems. In each case, punched discs provide laboratory-scale electrode samples that can be compared under testing or incorporated into device assemblies. The method is especially useful when researchers need consistent sample geometry to evaluate electrode formulations, calculate area-based quantities, and connect fabrication quality with electrochemical performance.