Stack-and-draw Technique

The stack-and-draw technique is a fabrication method that converts an arrangement of glass rods and capillaries into a continuous fiber with a controlled internal structure. In optical-fiber engineering, elements are stacked to form a preform, heated until the glass softens, and drawn to reduce its diameter while preserving the relative geometry of the components; repeated drawing can further refine the structure. This process enables precise control of hollow channels, cladding features, and core configurations in microstructured and photonic-crystal fibers. The resulting fibers support tailored light guidance and are used in telecommunications, sensing, nonlinear optics, and other applications requiring specialized optical properties.

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JoVE Business - Microeconomics

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2025

The Rawlsian social welfare function, introduced by philosopher John Rawls, is a framework in welfare economics that assesses societal well-being by focusing on the utility of the least advantaged members. This approach aligns with Rawls's theory of justice, which posits that a just society should prioritize improving the conditions of its most disadvantaged individuals.Consider a society with three individuals:Individual A: Utility level of 8Individual B: Utility level of 4Individual C:...

Research

JoVE Journal - Bioengineering

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Cited by 7 •

2017

Scalable engineered blood vessels would improve clinical applicability. Using easily sizable 3D-printed guides, rings of vascular smooth muscle were created and stacked into a tubular form, forming a vascular graft. Grafts can be sized to meet the range of human coronary artery dimensions by simply changing the 3D-printed guide size.

Research

JoVE Journal - Biology
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Enhancing Density Maps by Removing the Majority of Particles in Single Particle Cryogenic Electron Microscopy Final Stacks

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Cited by 1 •

2024

An advanced particle selection method for cryo-EM, namely CryoSieve, improves density map resolution by removing a majority of particles in final stacks, as demonstrated through its application on a real-world dataset.

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JoVE Journal - Immunology and Infection

Production of Adeno-Associated Virus Vectors in Cell Stacks for Preclinical Studies in Large Animal Models

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Cited by 17 •

2021

Here we provide a detailed procedure for large-scale production of research-grade AAV vectors using adherent HEK 293 cells grown in cell stacks and affinity chromatography purification. This protocol consistently yields >1 x 1013 vector genomes/mL, providing vector quantities appropriate for large animal studies.

Advanced Animal Model of Colorectal Metastasis in Liver: Imaging Techniques and Properties of Metastatic Clones

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Cited by 6 •

2016

The ability of metastatic clones to colonize distant sites depends on their proliferation capacity and/or their ability to survive in the host microenvironment without significant proliferation. Here, we present an animal model that allows quantitative visualization of both types of liver colonization by metastatic clones.

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