Dynamic Flow Bioreactor
Dynamic Flow Bioreactor - Related Videos
12:54
Operation of a Benchtop Bioreactor
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Cited by 9 •
2013
Fermentors are used to increase culture yield and productivity of bioengineered cells. After screening multiple microbial or animal cell culture candidates in shake flasks, the next logical step is to increase the selected culture’s biomass with the fermentor. This video demonstrates the setup and operation of a typical benchtop bioreactor system.
09:40
Batch and Continuous Bioreactors
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2023
Bioreactors are used to grow organisms in large volumes, thereby enabling the production of mass quantities of the target product. These reactors can be batch reactors, which contain all of the components needed for cell growth, or continuous reactors, which have inlet and outlet ports allowing for the addition of fresh growth media and the removal of cell waste. This video presents batch and continuous reactors and demonstrates the use of bioreactors to grow bacteria in the laboratory.
11:22
Engineering Biological-Based Vascular Grafts Using a Pulsatile Bioreactor
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Cited by 31 •
2011
Our group has developed a bioreactor culture system that mimics the physiological pulsatile stresses of the cardiovascular system to regenerate implantable small-diameter vascular grafts.
04:17
Ex Vivo Placental Explant Flow Culture - Mimicking the Dynamic Conditions In Utero
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Cited by 2 •
2023
Here is a protocol for culturing placental explants under constant flow conditions. This approach enhances traditional static villous culture systems by enabling the replication of dynamic physiological environments.
06:44
Ex Vivo Perfusion Culture of Large Blood Vessels in a 3D Printed Bioreactor
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Cited by 1 •
2023
This protocol presents the setup and operation of a newly developed, 3D printed bioreactor for the ex vivo culture of blood vessels in perfusion. The system is designed to be easily adopted by other users, practical, affordable, and adaptable to different experimental applications, such as basic biology and pharmacological studies.
FAQs
Flow rate determines how quickly culture medium reaches the biological material, while shear stress supplies a mechanical cue produced by fluid movement. Together, these variables can alter cell behavior and tissue development: insufficient transport may limit delivery, whereas excessive mechanical stimulation may change the intended culture environment. Careful control links fluid handling to engineered tissue performance.
Pumps or other flow mechanisms establish movement that circulates medium continuously or periodically around the cultured material. This circulation helps distribute nutrients and oxygenation conditions through the system and supports more uniform exposure to the intended environment. The selected flow pattern therefore affects mass transport, cell responses, and consistency during tissue or product development.
Flow rate alone does not describe the culture environment. Nutrient delivery and oxygenation determine whether cells receive key resources, while fluid movement influences how effectively those resources reach three-dimensional materials. Managing these factors together is important when the goal is organized tissue development, functional performance, or reproducible biological products.
Shear stress can change cell behavior because moving fluid imposes a physical force on cells and developing tissue. Its effect is linked to flow rate and the biological material being cultured. Controlling this force helps researchers apply mechanical cues that better match the intended tissue-development environment and can support improved organization or functional performance.
A practical setup requires a biological material or scaffold, culture medium, and a pump or another mechanism that controls circulation. Researchers also regulate flow rate, nutrient delivery, oxygenation, and the resulting shear stress. Keeping these conditions controlled allows the system to provide repeatable physical and biochemical cues during three-dimensional culture or scaffold maturation.
A typical workflow places cells or other biological material in a three-dimensional culture or scaffold, connects the culture to a pump-driven circulation system, and establishes the desired flow conditions. Researchers then use controlled medium movement to support nutrient and oxygen delivery while applying mechanical cues during tissue development or scaffold maturation.
These systems are useful when a project requires three-dimensional cell culture, scaffold maturation, organ or tissue models, or bioprocess development. They are especially relevant when researchers need to combine improved mass transport with controlled mechanical cues. The approach can therefore support both experimental tissue models and the development of engineered biological products.
Researchers can assess whether controlled fluid movement improves tissue organization, functional performance, or maturation of a scaffold-based construct. The systems also contribute to reproducibility in engineered biological products by making flow, transport, and mechanical conditions more controllable. In bioengineering, these outcomes help connect culture conditions with the quality and behavior of developing tissues or models.