Spectral Acquisition

Spectral acquisition is the process of measuring and recording signal intensity across wavelengths or frequencies to characterize how a material, device, or environment interacts with electromagnetic radiation. In engineering, an instrument separates the incoming signal into spectral components, directs them to a detector, and converts the response into a calibrated spectrum whose resolution and range depend on the optics, sensor, and acquisition settings. These data support material identification, chemical and process monitoring, fault detection, remote sensing, and performance evaluation of optical and electronic systems, while careful calibration and noise control improve measurement reliability and enable comparison across experiments.

Spectral Acquisition - Related Videos

Research

JoVE Journal - Immunology and Infection

High-plex Imaging using Spectral Confocal Microscopy to Minimize Non-specific Tissue Fluorescence

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Cited by 1 •

2025

Spectral Iterative Bleaching Extends Multiplexity (IBEX) builds upon the base IBEX technique by adding heparin blocking to minimize nonspecific binding and leveraging spectral detection with computational unmixing to suppress autofluorescence. This approach accelerates image acquisition while reducing sources of background, enabling robust multi-round, high-parameter spatial proteomic analyses.

Using Spectral Reflectometry to Determine Myelinated Axon Diameters in a Fixed Mouse Brain Slice

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2025

Source: Kwon, J., et al., Spectral Reflectometric Microscopy on Myelinated Axons In Situ. J. Vis. Exp. (2018) This video demonstrates the use of spectral reflectometry with a hyperspectral confocal microscope to analyze interference patterns and determine myelinated axon diameters in fixed brain tissue slices.

Integrated Photoacoustic Ophthalmoscopy and Spectral-domain Optical Coherence Tomography

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Cited by 24 •

2013

Photoacoustic ophthalmology (PAOM), an optical-absorption-based imaging modality, provides the complementary evaluation of the retina to the currently available ophthalmic imaging technologies. We report the using of PAOM integrated with spectral-domain optical coherence tomography (SD-OCT) for simultaneous multimodal retinal imaging in rats.

Research

JoVE Journal - Chemistry
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ARL Spectral Fitting as an Application to Augment Spectral Data via Franck-Condon Lineshape Analysis and Color Analysis

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Cited by 1 •

2021

This protocol introduces Franck-Condon Lineshape Analyses (FCLSA) of emission spectra and serves as a tutorial for the use of ARL Spectral Fitting software. The open-source software provides an easy and intuitive way to perform advanced analysis of emission spectra including excited state energy calculations, CIE color coordinate determination, and FCLSA.

In vivo Quantification of G Protein Coupled Receptor Interactions using Spectrally Resolved Two-photon Microscopy

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Cited by 6 •

2011

By employing a spectrally resolved two-photon microscopy imaging system, pixel-level maps of Förster Resonance Energy Transfer (FRET) efficiencies are obtained for cells expressing membrane receptors hypothesized to form homo-oligomeric complexes. From the FRET efficiency maps, we are able to estimate stoichiometric information about the oligomer complex under study.

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