Fluid Shear Flow
Fluid Shear Flow - Related Videos
09:20
The Assembly and Application of 'Shear Rings': A Novel Endothelial Model for Orbital, Unidirectional and Periodic Fluid Flow and Shear Stress
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Cited by 10 •
2016
Different levels and patterns of fluid shear are known to modulate endothelial gene expression, phenotype and susceptibility to disease. We discuss the assembly and use of 'shear rings': a model that produces unidirectional, periodic shear stress patterns. Shear rings are simple to assemble, economical and can produce high cell yields.
12:43
Parallel-plate Flow Chamber and Continuous Flow Circuit to Evaluate Endothelial Progenitor Cells under Laminar Flow Shear Stress
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Cited by 51 •
2012
We are describing a method to subject adherent cells to laminar flow shear stress in a sterile continuous flow circuit. The cells' adhesion, morphology can be studied through the transparent chamber, samples obtained from the circuit for metabolite analysis and cells harvested after shear exposure for future experiments or culture.
07:26
Agarose Fluid Gels Formed by Shear Processing During Gelation for Suspended 3D Bioprinting
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Cited by 6 •
2023
Shear processing during hydrogel formation results in the production of microgel suspensions that shear-thin but rapidly restructure following the removal of shear forces. Such materials have been used as a supporting matrix for bioprinting complex, cell-laden structures. Here, methods used to manufacture the supporting bed and compatible bioinks are described.
10:28
Experimental Measurement of Settling Velocity of Spherical Particles in Unconfined and Confined Surfactant-based Shear Thinning Viscoelastic Fluids
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Cited by 15 •
2014
This paper demonstrates the experimental procedure to measure terminal settling velocities of spherical particles in surfactant-based shear thinning viscoelastic fluids. Fluids over a wide range of rheological properties are prepared and settling velocities are measured for a range of particle sizes in unbounded fluids and fluids between parallel walls.
11:38
Visualization and Quantification of TGFβ/BMP/SMAD Signaling under Different Fluid Shear Stress Conditions using Proximity-Ligation-Assay
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Cited by 4 •
2021
Here, we establish a protocol to simultaneously visualize and analyze multiple SMAD complexes using proximity ligation assay (PLA) in endothelial cells exposed to pathological and physiological fluid shear stress conditions.
FAQs
Shear stress increases when neighboring fluid layers have a greater difference in velocity across a given distance. Viscosity determines how strongly the fluid resists this relative motion, so the same velocity gradient can produce different forces in different fluids. This relationship helps researchers set conditions that reproduce desired mechanical environments in bioengineering systems.
Flow rate, fluid viscosity, and channel geometry are central variables because they shape the velocity gradients and resulting forces at surfaces or interfaces. Changing any of them can alter the mechanical environment experienced by cells or engineered materials. Controlling these parameters allows researchers to compare experiments consistently and tune conditions for specific biological or device-related goals.
Shear stress acts parallel to a surface or interface, exposing attached cells to directional mechanical forces rather than only normal pressure. In bioengineering studies, those forces can influence cell adhesion, alignment, growth, and differentiation. Recognizing this directional effect is important when evaluating how cells respond to flow-based environments or when designing systems intended to guide tissue organization.
A practical workflow begins by specifying the fluid, flow rate, and channel or chamber geometry, then assessing the resulting velocity gradients and shear stress. Researchers can use that characterization to determine whether the system represents the intended mechanical environment. Establishing these conditions before biological testing improves interpretation of cell or tissue responses.
It is useful when a microfluidic system must expose cells, materials, or interfaces to controlled mechanical forces. By relating flow rate, viscosity, and channel geometry to shear conditions, designers can adjust the environment within the device. This supports systems that model aspects of blood circulation, investigate cell responses, or evaluate biomedical device performance.
In blood-circulation models, controlled shear conditions help reproduce a mechanically relevant flow environment. In bioreactors, the same principle enables researchers to regulate forces experienced by cells or engineered tissues. Measurements of shear stress can then support evaluation of tissue behavior and refinement of cell-based systems, including conditions related to adhesion, growth, alignment, and differentiation.