Eye Squint Quantification

Eye squint quantification is the measurement of eyelid narrowing to convert changes in eye openness into objective behavioral data. It typically uses images or video to assess eyelid aperture, the distance between upper and lower eyelids, or the position of facial landmarks, allowing squint intensity and duration to be scored across observations. In behavioral research, these measurements help characterize responses to visual stimuli, discomfort, stress, pain, social cues, or changing environmental conditions. Standardized quantification reduces reliance on subjective ratings and supports comparisons among individuals, experimental conditions, and time points, strengthening the analysis of facial behavior in laboratory and field studies.

Eye Squint Quantification - Related Videos

Research

JoVE Journal - Behavior

An Automated Squint Method for Time-syncing Behavior and Brain Dynamics in Mouse Pain Studies

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Cited by 2 •

2024

This protocol provides a method for tracking automated eye squint in rodents over time in a manner compatible with time-locking to neurophysiological measures. This protocol is expected to be useful to researchers studying mechanisms of pain disorders such as migraine.

Quantification of Tear Volume in an Autoimmune Dry Eye Rat Model using the Phenol Red Thread

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2025

This video demonstrates the phenol red thread test to determine tear volume in an autoimmune dry eye rat model. The pH-sensitive acidic phenol red thread changes color when it comes in contact with the alkaline tears. The shorter length of color change in the phenol red thread corresponds to reduced tear volume.

Research

JoVE Journal - Medicine

Quantification of Diabetes-induced Adherent Leukocytes in Retinal Vasculature

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2025

We demonstrate a method to label the walls of the retinal vasculature and adherent leukocytes. These adherent leukocytes can then be counted under a fluorescence microscope as a parameter of inflammation or the response of that inflammation to therapies.

TD-DFT Guided Advanced E-Eye Sensing Technique for On-site Quantification of Fe, Cr, F, and As in the Environmental, Biological, and Food Samples

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2025

This work has discussed the protocol for designing and developing an E-Eye-Enable POCT device to detect Fe, Cr, As, and F in environmental samples, biological samples, and food and beverages. Nearly 2000 samples have been tested, and the results accord with the gold-standard technique.

A Rat Dry Eye Model with Lacrimal Gland Dysfunction Induced by Scopolamine

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Cited by 3 •

2024

Here, we establish a rat model of lacrimal gland dysfunction to provide a basis for the study of aqueous-deficient dry eye.

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