Blue Light Crosslinking
Blue Light Crosslinking - Related Videos
02:52
Blue Light Therapy for Drug-Resistant Bacterial Infections in Burn Wounds in a Mouse Model
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2026
Source: Wang, Y., et al. In Vivo Investigation of Antimicrobial Blue Light Therapy for Multidrug-resistant Acinetobacter baumannii Burn Infections Using Bioluminescence Imaging. J. Vis. Exp. (2017)This video demonstrates the procedure for treating burn wound infections caused by bioluminescent, drug-resistant bacteria in mice using blue light therapy, followed by bioluminescence imaging to monitor treatment efficacy.
03:47
Crosslinking Neuronal Surface Proteins in the Mouse Brain using a Chemical Crosslinking Assay
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2025
This video demonstrates the crosslinking of neuronal surface proteins using Bis-sulfosuccinimidyl suberate or BS3. Isolated and chilled mouse brain is sectioned coronally. The desired region is extracted from the sections, minced, and treated with BS3. BS3 crosslinks surface proteins like GABA receptors, leaving internal proteins unaltered. The reaction is quenched with glycine before processing the samples for further analysis.
11:15
Nanosponge Tunability in Size and Crosslinking Density
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Cited by 1 •
2017
This article describes a process for tuning the size and crosslinking density of covalently crosslinked nanoparticles from linear polyesters containing pendant functionality. By tailoring synthesis parameters (polymer molecular weight, pendant functionality incorporation, and crosslinker equivalents), a desired nanoparticle size and crosslinking density can be achieved for drug delivery applications.
10:12
Quantitative Detection of DNA-Protein Crosslinks and Their Post-Translational Modifications
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Cited by 3 •
2023
The present protocol highlights a modified method to detect and quantify DNA-protein crosslinks (DPCs) and their post-translational modifications (PTMs), including ubiquitylation, SUMOylation, and ADP-ribosylation induced by topoisomerase inhibitors and by formaldehyde, thereby allowing the study of the formation and repair of DPCs and their PTMs.
09:06
Preparation of DNA-crosslinked Polyacrylamide Hydrogels
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Cited by 5 •
2014
Our laboratory has developed DNA-crosslinked polyacrylamide hydrogels, a dynamic hydrogel system, to better understand the effects of modulating tissue stiffness on cell function. Here, we provide schematics, descriptions, and protocols to prepare these hydrogels.
FAQs
A compatible photoinitiator acts as the light-responsive component. When it absorbs blue light, it generates reactive species that can link functional groups on neighboring polymer chains. This converts molecular-level activation into network formation, while the polymer chemistry determines whether the resulting material becomes a hydrogel or solid matrix. This distinction guides biomaterial and initiator selection.
The irradiation step provides spatial control because curing occurs where the blue light is applied. Researchers can therefore solidify selected regions rather than treating the entire material uniformly. This localized activation is useful for constructing defined shapes, patterned hydrogel features, or biofabricated structures whose geometry depends on where light reaches the precursor.
Stiffness becomes a design variable because crosslinking changes a soluble precursor into an interconnected polymer network. In bioengineering, researchers can use this controllable solidification to tailor matrices for tissue-engineering scaffolds, cell encapsulation, or delivery systems. The resulting mechanical differences help align the material with the intended construct design.
A basic workflow begins by preparing a liquid or soluble biomaterial with a compatible photoinitiator, positioning the material in the desired geometry, and irradiating it with visible blue light. The photoinitiator then generates reactive species, allowing neighboring polymer chains to link. This sequence produces the target hydrogel or solid matrix.
Blue Light Crosslinking is especially useful when a project requires rapid, spatially controlled solidification. Supported applications include hydrogel fabrication, cell encapsulation, tissue-engineering scaffolds, drug delivery systems, and biofabrication. Across these settings, the method helps translate a liquid precursor into a shaped construct, matrix, or carrier with reproducible placement.
Relatively mild operating conditions are valuable because they allow the method to support bioengineering applications such as cell encapsulation and tissue-engineering scaffold fabrication. When combined with rapid solidification and spatial control, they help researchers create constructs with defined shapes and curing locations, improving design control and reproducibility across hydrogel and biofabrication workflows.