Bovine Tail Disc
Bovine Tail Disc - Related Videos
13:37
Preparation of Intact Bovine Tail Intervertebral Discs for Organ Culture
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Cited by 34 •
2012
This protocol illustrates a harvesting technique for coccygeal bovine intervertebral discs for organ culture for in vitro organ culture.
10:17
The Tail Suspension Test
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Cited by 527 •
2012
The tail-suspension test is validated as an experimental procedure to assess antidepressant efficacy of drug treatments in mice. Mice are suspended by their tails for six minutes and escape-related behaviors are assessed. We describe procedures used in conducting the tail suspension test.
04:27
Generating a Pro-Inflammatory Organ Culture Model to Simulate an Early Intervertebral Disc Disease
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2025
This video demonstrates a technique for culturing bovine intervertebral discs in bioreactors to develop a three-dimensional organ culture model. Specific nutrient-deprived media and degenerative pressure conditions are applied to the discs, mimicking the pro-inflammatory and degenerative microenvironment observed during early-stage intervertebral disc disease. The disc heights are measured post-treatment to analyze the effects of nutrient deprivation and degenerative loading.
06:22
Optical Sectioning and Visualization of the Intervertebral Disc from Embryonic Development to Degeneration
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2021
We present a method to investigate spatial chondrocyte organization in the anulus fibrosus of the intervertebral disc using an optical sectioning method.
02:10
Generating an Organ Culture Model to Simulate Early-Stage Intervertebral Disc Disease
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2025
This video demonstrates the development of a pro-inflammatory and degenerative organ culture model for early-stage intervertebral disc (IVD) disease using TNF-α. It outlines the procedure for injecting TNF-α into the intervertebral disc and explains the mechanisms through which TNF-α induces inflammation and degradation of the IVD.
FAQs
The gelatinous nucleus pulposus and layered annulus fibrosus provide distinct structural features that researchers can examine together. Their arrangement allows studies to connect extracellular matrix composition with the disc’s mechanical response. This organization is useful for evaluating how tissue structure changes under loading and for assessing whether engineered or repaired constructs reproduce relevant disc behavior.
Extracellular matrix composition helps determine how disc tissue behaves mechanically and biologically. By examining this relationship, researchers can investigate how matrix features influence responses to mechanical loading and degeneration. In bioengineering, the same framework supports evaluation of biomaterials, engineered tissues, and cell-based therapies intended to restore or modify disc tissue properties.
The vertebral endplates provide an interface between the disc and adjacent vertebral tissue, allowing experiments to consider the disc within a more complete structural setting. Including this interface helps researchers examine disc behavior in relation to surrounding anatomy rather than focusing only on isolated internal regions, which is relevant to repair and organ culture studies.
Bovine tail discs can be incorporated into organ culture systems that maintain disc tissue for investigation under controlled conditions. These systems allow researchers to examine tissue behavior while testing variables such as mechanical loading, extracellular matrix influences, biomaterials, engineered tissues, or cell-based therapies. The resulting observations support controlled comparisons of disc responses during research.
This model supports evaluation of biomaterials, engineered tissues, and cell-based therapies designed for disc repair or regeneration. Researchers can examine how these interventions interact with disc structure, matrix composition, and mechanical behavior. Such testing helps identify whether a candidate approach produces outcomes relevant to restoring disc function before it informs broader regenerative strategy development.
Results from bovine tail disc studies can improve understanding of how disc structure, extracellular matrix composition, and mechanical loading relate to degeneration and repair. In bioengineering, those findings provide a basis for developing regenerative strategies and evaluating candidate interventions. They can therefore guide research addressing mechanisms and treatment concepts relevant to human intervertebral disc disease.