Neonatal Heart Scaffold
Neonatal Heart Scaffold - Related Videos
08:16
Neonatal Cardiac Scaffolds: Novel Matrices for Regenerative Studies
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Cited by 3 •
2016
In these studies, we provide methodology for novel, neonatal, murine cardiac scaffolds for use in regenerative studies.
08:23
Modified Technique for the Use of Neonatal Murine Hearts in the Langendorff Preparation
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Cited by 1 •
2022
The present protocol describes aortic cannulation and retrograde perfusion of the ex-vivo neonatal murine heart. A two-person strategy, using a dissecting microscope and a blunted small gauge needle, permits reliable cannulation. Quantification of longitudinal contractile tension is achieved using a force transducer connected to the apex of the left ventricle.
03:58
Apical Resection Neonatal Mouse Model: A Surgical Procedure to Amputate the Ventricular Apex to Study Regenerative Potential in a Neonate Mouse Heart
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2025
In this video, we demonstrate the procedure to surgically remove the left ventricular apex of a neonatal mouse heart.
09:31
Hemodynamic Precision in the Neonatal Intensive Care Unit using Targeted Neonatal Echocardiography
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Cited by 6 •
2023
Presented here is a protocol to perform comprehensive neonatal echocardiography by trained neonatologists in the neonatal intensive care unit. The trained individuals provide longitudinal assessments of heart function, systemic and pulmonary hemodynamics in a consultative role. The manuscript also describes the requirements to become a fully trained neonatal hemodynamics specialist.
10:05
Anatomically Realistic Neonatal Heart Model for Use in Neonatal Patient Simulators
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Cited by 7 •
2019
This protocol describes a procedure for creating functional artificial neonatal heart models by utilizing a combination of magnetic resonance imaging, 3D printing, and injection molding. The purpose of these models is for integration into the next generation of neonatal patient simulators and as a tool for physiological and anatomical studies.
FAQs
The scaffold’s three-dimensional architecture provides spatial cues that influence how cardiac cells attach, organize, and form tissue. Its geometry must support tissue development without preventing the heart from adapting as it grows. In bioengineering studies, adjusting this architecture helps researchers examine how physical structure affects organization and repair of neonatal myocardium.
Stiffness affects how cells interact with the scaffold and how the developing tissue is physically supported. A suitable level must balance stability with biological compatibility, because excessive structural resistance could hinder adaptation while insufficient support may not adequately guide tissue formation. This makes stiffness central to designing scaffolds for an immature, growing heart.
Porosity creates pathways through the scaffold that support the movement of nutrients and oxygen. These transport properties are important because developing cardiac tissue depends on access to its surrounding environment as cells attach and organize. Consequently, porosity must be considered alongside architecture and stiffness when evaluating whether a scaffold can support tissue formation.
Gradual material degradation can allow the scaffold to provide temporary support while newly developing tissue takes on a greater structural role. For neonatal applications, the timing of this transition matters because the heart continues to grow and adapt. Scaffold design therefore aims to balance persistence for support with gradual replacement by developing tissue.
These scaffolds provide a controlled three-dimensional setting for investigating how cardiac tissue organizes and develops. Researchers can use the platform to examine the relationship between biomaterial properties, cell attachment, tissue formation, and transport of nutrients and oxygen. This helps connect physical scaffold design with processes relevant to the developing neonatal heart.
A scaffold can serve as a bioengineering platform for modeling congenital heart disease by providing a structured environment in which cardiac tissue development can be examined. Its architecture, stiffness, porosity, and degradation behavior offer design variables for studying how altered physical conditions may affect organization and growth in developing heart tissue.
Regenerative approaches use these scaffolds to support investigation of damaged or underdeveloped myocardium. The framework may guide cell attachment and tissue formation while supplying structural support and pathways for nutrient and oxygen transport. Because the neonatal heart is still growing, successful designs must also accommodate changing tissue demands rather than remain biologically static.