Biotinylated Dextrin Amine
Biotinylated Dextrin Amine - Related Videos
13:12
Transsynaptic Tracing from Peripheral Targets with Pseudorabies Virus Followed by Cholera Toxin and Biotinylated Dextran Amines Double Labeling
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Cited by 8 •
2015
Transsynaptic tracing has become a powerful tool for analyzing central efferents regulating peripheral targets through multi-synaptic circuits. Here we present a protocol that exploits the transsynaptic pseudorabies virus to identify and localize a functional brain circuit, followed by classical tract tracing techniques to validate specific connections in the circuit between identified groups of neurons.
06:39
Improving the Application of High Molecular Weight Biotinylated Dextran Amine for Thalamocortical Projection Tracing in the Rat
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2018
Here, we present a refined protocol to effectively reveal biotinylated dextran amine (BDA) labeling with a fluorescent staining method through a reciprocal neural pathway. It is suitable for analyzing the fine structure of BDA labeling and distinguishing it from other neural elements under a confocal laser scanning microscope.
09:13
Cell-surface Biotinylation Assay
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2023
A cell can regulate the amount of particular proteins on its cell membrane through endocytosis, following which cell surface proteins are effectively sequestered in the cytoplasm. Once within a cell, these surface proteins can be either destroyed or “recycled” back to the membrane. The cell surface biotinylation assay provides researchers with a way to study these phenomena. The technique makes use of a derivative of the small molecule biotin, which can label surface proteins and then be...
01:19
Amines to Amides: Acylation of Amines
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2025
Various carboxylic acid derivatives (such as acid chlorides, esters, and anhydrides) can be used for the acylation of amines to yield amides. The reaction requires two equivalents of amines. The first amine molecule functions as a nucleophile and attacks the carbonyl carbon to produce a tetrahedral intermediate. This is followed by the loss of the leaving group and restoration of the C=O bond. Next, the second equivalent of amine serves as a Brønsted base and deprotonates the quaternary amide...
02:56
FITC-Dextran Feeding: A Method to Quantify Intestinal Permeability in C. elegans
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2023
This video introduces a method of to visualize and measure the integrity of the C. elegans intestine lumen by feeding worms FITC-labeled dextran. The example protocol shows the assay done with 3,3’-diindolylmethane (DIM)-treated and pathogen-fed worms.
FAQs
The amine-linked dextrin forms the transported polymeric component, allowing labeled material to move along neuronal axons after uptake. Biotin provides a separate detection handle rather than determining transport itself. Avidin or streptavidin conjugated to fluorescent or enzymatic labels binds the biotin groups, converting transported material into a visible signal for examining neural pathways in fixed tissue.
The direction of labeling depends on the experimental conditions under which neurons take up and transport the tracer. Anterograde labeling follows movement from neuronal cell bodies toward their axonal targets, whereas retrograde labeling can identify projections returning toward source neurons when the relevant conditions support that transport. Interpretation therefore requires attention to labeling direction and experimental design.
Avidin and streptavidin conjugates recognize the tracer's biotin groups and carry the signal used for visualization. Fluorescent conjugates can reveal labeled structures through fluorescence-based imaging, while enzymatic conjugates generate a detectable reaction product. This detection step makes the transported polymer visible and allows researchers to distinguish pathway organization and terminal distributions in fixed brain tissue.
The resulting labeling pattern can show how a pathway is organized, where axons terminate, and which regions are structurally connected. Terminal fields are especially informative because they indicate the distribution of axonal endings within a target region. Examining these spatial patterns helps relate anatomical connectivity to the structural organization of neural circuits.
A typical workflow begins with neuronal uptake of the tracer, followed by transport of the amine-linked polymer along axons. Tissue is then examined after labeling is visualized through avidin or streptavidin linked to a fluorescent or enzymatic marker. Researchers interpret the resulting distribution by assessing labeled pathways, projection direction, and terminal fields across fixed brain tissue.
Biotinylated Dextrin Amine is useful when researchers need anatomical evidence of connections between nervous-system regions. Labeling can support comparisons across development, after injury, or following experimental manipulation, allowing investigators to examine connectivity changes rather than only the presence of a pathway. These results provide structural context for studies linking neural circuits with behavior.