Minute Pirate Bugs

Minute pirate bugs are small predatory true bugs in the genus Orius that help regulate insect populations in gardens, agricultural systems, and natural environments. Using piercing-sucking mouthparts, they locate prey such as thrips, aphids, mites, and insect eggs, then inject digestive enzymes and consume the liquefied contents; they also feed on pollen and plant fluids when prey is scarce. Their broad diet and active hunting make them valuable agents of biological control, reducing reliance on chemical pesticides and supporting integrated pest management. Studying their behavior, habitat needs, and seasonal abundance helps researchers evaluate biodiversity and strengthen environmentally sustainable crop protection.

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Research

JoVE Journal - Developmental Biology

Targeted Microinjection and Electroporation of Primate Cerebral Organoids for Genetic Modification

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Cited by 5 •

2023

The electroporation of primate cerebral organoids provides a precise and efficient approach to introduce transient genetic modification(s) into different progenitor types and neurons in a model system close to primate (patho)physiological neocortex development. This allows the study of neurodevelopmental and evolutionary processes and can also be applied for disease modeling.

A Neural Implant Design Toolbox for Nonhuman Primates

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Cited by 2 •

2024

This paper outlines automated processes for nonhuman primate neurosurgical planning based on magnetic resonance imaging (MRI) scans. These techniques use procedural steps in programming and design platforms to support customized implant design for NHPs. The validity of each component can then be confirmed using three-dimensional (3D) printed life-size anatomical models.

Research

JoVE Journal - Neuroscience
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DiOLISTIC Labeling of Neurons from Rodent and Non-human Primate Brain Slices

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Cited by 60 •

2010

We demonstrate the use of the gene gun to introduce fluorescent dyes, such as DiI, into neurons in brain slices from rodents and non-human primates of different ages. In this particular case, we use adult mice (3-6 months old) and adult cynomologus monkeys (9-15 years old). This technique, originally described by the laboratory of Dr. Lichtman (Gan et al., 2000), is well suited for the study of dendritic branching and dendritic spine morphology and can be combined with traditional...

A Method for Evaluating Insecticide Efficacy against Bed Bug, Cimex lectularius, Eggs and First Instars

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Cited by 5 •

2017

Insecticide evaluations are often targeted against adult insects, rather than immature stages. Here, we present a protocol for evaluating insecticides against bed bug eggs with a comparison to the first nymphal bed bug stage. These protocols could be adjusted for other insects to evaluate insecticide efficacy in non-adult life stages.

Using Single Sensillum Recording to Detect Olfactory Neuron Responses of Bed Bugs to Semiochemicals

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Cited by 3 •

2016

Bed bugs rely on olfactory receptor neurons housed in their antennal olfactory sensilla to detect semiochemicals in the environment. Utilizing single sensillum recording, we demonstrate a method to evaluate bed bug response to semiochemicals and explore the coding process involved.

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