Mouse Stroke Model
Mouse Stroke Model - Related Videos
10:45
The Mouse Stroke Unit Protocol with Standardized Neurological Scoring for Translational Mouse Stroke Studies
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2025
This article presents a protocol for executing the highly translational and independently proven mouse Stroke Unit (mSU) concept in large-stroke mice. It also provides a standardized protocol for executing the focal experimental stroke scale in mice, which evaluates stroke focal deficits detected even long-term.
02:59
Stereotactic Injection of Hydrogel into a Post-Stroke Mouse Model
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2025
Source: Wilson, K.L., et al. Injection of Hydrogel Biomaterial Scaffolds to The Brain After Stroke. J. Vis. Exp. (2020)This video demonstrates the stereotaxic injection of a hydrogel into the stroke core of a post-stroke mouse, highlighting its role in scaffold formation that mimics the extracellular matrix, promotes cellular infiltration, and supports angiogenesis, underscoring its neurotherapeutic potential in stroke recovery.
04:32
Exertional Heat Stroke Mouse Model: A Protocol to Study Mechanisms Underlying Exertional Heat Stroke
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2025
In this video we describe a step-by-step protocol to study exertional heat stroke (EHS) in a mouse model.
02:42
Inducing Thrombotic Stroke Through Hypoxia and Ischemia in a Mouse Model
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2025
This video demonstrates the procedure of replicating a thrombotic stroke in a mouse model by blocking the right common carotid artery and exposing the mouse to a low-oxygen environment. This process reduces blood and oxygen flow to the brain, causing blood clot formation and brain damage.
08:14
Modeling Stroke in Mice: Permanent Coagulation of the Distal Middle Cerebral Artery
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Cited by 103 •
2014
Various murine models of middle cerebral artery occlusion (MCAO) are widely used in experimental brain research. Here, we demonstrate the model of transcranial permanent distal MCAO which produces consistent cortical infarction of a size corresponding to damage imposed by the majority of human ischemic strokes.
FAQs
In a middle cerebral artery occlusion experiment, reduced blood flow produces ischemia, infarction, and neurological deficits. When the occlusion is temporary, restoring circulation introduces reperfusion injury in addition to the initial ischemic damage. This design therefore lets investigators examine both the effects of interrupted blood flow and the consequences of its restoration.
Mouse stroke models can represent either ischemic or hemorrhagic injury, so the experimental mechanism determines the pathology being examined. Ischemic designs focus on consequences of blocked circulation, whereas hemorrhagic designs address bleeding-related brain injury. Selecting between them should match the clinical process, biological question, and outcomes the study intends to measure.
After the initial vascular event, these models support analysis of several linked responses, including inflammation, vascular changes, and neural repair. Studying them across the course of injury can show how the brain responds after damage rather than limiting evaluation to infarction alone. That broader view is useful when investigating recovery or neuroprotective strategies.
Treatment response is not inferred from a single observation. Mouse stroke studies can combine behavioral assessments with tissue-level measurements, allowing functional changes to be considered alongside brain injury. This paired interpretation helps investigators judge whether a candidate intervention affects behavior, tissue damage, recovery, or several outcomes at once.
At a high level, a study establishes the selected stroke injury, maintains the intended occlusion when required, and, in a transient design, restores circulation. Investigators then evaluate neurological deficits and collect behavioral or tissue-level outcomes. Keeping injury induction, reperfusion status, and assessment aligned with the study question supports interpretable comparisons.
The model serves as a preclinical platform for evaluating candidate therapies within a controlled experimental setting. Investigators can examine neuroprotection, inflammatory responses, vascular effects, or neural repair, while also assessing behavioral and tissue-level outcomes. Results may reveal factors associated with differing treatment responses, helping guide comparisons among interventions and later study design.