Fusion Pore Closure
Fusion Pore Closure - Related Videos
12:30
Confocal Microscopy to Measure Three Modes of Fusion Pore Dynamics in Adrenal Chromaffin Cells
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Cited by 1 •
2022
This protocol describes a confocal imaging technique to detect three fusion modes in bovine adrenal chromaffin cells. These fusion modes include 1) close-fusion (also called kiss-and-run), involving fusion pore opening and closure, 2) stay-fusion, involving fusion pore opening and maintaining the opened pore, and 3) shrink-fusion, involving fused vesicle shrinkage.
07:37
Neural Tube Closure in Mouse Whole Embryo Culture
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A method allowing for direct pharmacological manipulation of mouse embryos during neurulation that bypasses maternal metabolism is described. The technique can be adapted to study different aspects of neurulation by varying the time point and pharmacological agent.
02:45
Nuclear Fusion
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2020
The process of converting very light nuclei into heavier nuclei is also accompanied by the conversion of mass into large amounts of energy, a process called fusion. The principal source of energy in the sun is a net fusion reaction in which four hydrogen nuclei fuse and ultimately produce one helium nucleus and two positrons. A helium nucleus has a mass that is 0.7% less than that of four hydrogen nuclei; this lost mass is converted into energy during the fusion. This reaction produces about...
10:52
Closure of a Patent Foramen Ovale (PFO): An Intervention Sequence
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Cited by 1 •
2022
The closure of a patent foramen ovale (PFO) is a catheter-based intervention to prevent PFO-associated stroke. The PFO-occlusion device is advanced through the femoral vein and deployed across the interatrial septum using transesophageal echocardiography (TEE) and fluoroscopic guidance. The following protocol provides a step-by-step guide for the PFO-closure intervention using a double-disc device.
09:19
Analysis of SNARE-mediated Membrane Fusion Using an Enzymatic Cell Fusion Assay
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We have developed a cell fusion assay that quantifies SNARE-mediated membrane fusion events by activated expression of β-galactosidase.
FAQs
Closure is influenced by membrane curvature and tension at the narrow connection between the vesicle and cell surface. As these physical properties change, the pore can become more stable or move toward sealing. The coordinated activity of fusion proteins, including synaptotagmin and SNARE complexes, also contributes to this transition and affects how long the opening remains available for cargo release.
Synaptotagmin and SNARE complexes participate in the coordinated protein activity that shapes the pore’s transition toward closure. Their action is considered alongside membrane curvature and tension rather than as an isolated protein event. This coordination matters because the resulting pore behavior determines whether a vesicle releases only part of its contents or proceeds toward complete discharge.
A short-lived opening can permit brief, partial neurotransmitter release, whereas continued pore opening allows more complete discharge into the synaptic cleft. These alternatives change the amount of signaling material delivered during exocytosis. Consequently, fusion pore behavior provides a physical mechanism linking vesicle membrane dynamics with differences in synaptic strength.
The outcome depends on the interaction between membrane curvature, membrane tension, and fusion-protein coordination. Changes in any of these features can influence the pore’s stability and its progression toward sealing. In neuronal exocytosis, those differences affect the duration and extent of cargo release, making pore closure a regulated step rather than a uniform endpoint.
Examining pore dynamics can show whether neurotransmitter release is brief and partial or progresses to complete vesicle discharge. These observations help connect membrane events with synaptic strength and provide insight into how vesicles return to a recyclable state after exocytosis. The same analysis can also clarify how altered release behavior may relate to neurological disorders.
Closure links the end of neurotransmitter release with the later handling of the vesicle membrane, making it relevant to vesicle recycling in neurons. If exocytosis is altered, changes in pore behavior may affect synaptic signaling and recycling efficiency. Studying this relationship therefore helps place membrane fusion defects in the broader context of neurological disorders.