Paper-based Diagnostics
Paper-based Diagnostics - Related Videos
04:48
A Paper-Based Immunoassay for the Detection of Immunoglobulin G
0 Views •
2025
The video demonstrates a paper-based immunoassay, involving amine-functionalized cellulose discs cross-linked with glutaraldehyde. Covalent immobilization of IgG-Fc-specific capture antibodies, followed by the introduction of immunoglobulin G and horseradish peroxidase-conjugated detection antibodies, results in a color change confirming the presence of immunoglobulin G.
10:42
Design to Implementation Study for Development and Patient Validation of Paper-Based Toehold Switch Diagnostics
0 Views •
Cited by 6 •
2022
Access to decentralized, low-cost, and high-capacity diagnostics that can be deployed into the community for decentralized testing is critical for combating global health crises. This manuscript describes how to build paper-based diagnostics for viral RNA sequences that can be detected with a portable optical reader.
01:30
Reading Research Papers
0 Views •
2026
Research articles are structured documents designed to communicate scientific findings efficiently and transparently. Rather than reading them sequentially like a narrative, readers can approach them strategically by understanding their standardized organization. Evaluating the title, authors, and institutional affiliations provides an initial indication of relevance, expertise, and credibility. The Abstract offers a concise summary of the study’s objective, methodology, and main findings,...
11:33
Fabrication of Three-dimensional Paper-based Microfluidic Devices for Immunoassays
0 Views •
Cited by 13 •
2017
We detail a method to fabricate three-dimensional paper-based microfluidic devices for use in the development of immunoassays. Our approach to device assembly is a type of multilayer, additive manufacturing. We demonstrate a sandwich immunoassay to provide representative results for these types of paper-based devices.
11:53
Paper-based Devices for Isolation and Characterization of Extracellular Vesicles
0 Views •
Cited by 40 •
2015
This protocol details a method to isolate extracellular vesicles (EVs), small membranous particles released from cells, from as little as 10 μl serum samples. This approach circumvents the need for ultracentrifugation, requires only a few minutes of assay time, and enables the isolation of EVs from samples of limited volumes.
FAQs
Fluid transport occurs as the sample moves through the paper’s porous structure by capillary action, without requiring an external pump. Patterned channels help direct that movement toward regions containing reagents. This combination determines where the sample travels and where the target interaction occurs, supporting compact testing formats that need only small sample volumes.
Embedded reagents interact with the biological or chemical target as the sample reaches the designated testing region. That interaction produces a visible color change or another measurable signal. The reagents therefore connect target recognition to result generation, allowing the device to indicate whether the analyte is present and, when measurable signals are used, support assessment of its amount.
Patterned channels organize fluid flow across the paper and guide samples to specific reagent-containing zones. This spatial arrangement helps separate the movement of the sample from the location where detection occurs, while preserving the device’s compact format. In bioengineering designs, channel patterns contribute to portability and reduce the need for complex external equipment.
A typical workflow begins when a sample is placed on the paper device. Capillary action carries it through patterned channels until it contacts embedded reagents. The target-reagent interaction then produces a visible color change or another measurable signal, which provides the test result. This sequence enables analysis with minimal equipment and a small sample volume.
They are particularly useful when testing must occur outside a centralized laboratory, including resource-limited settings and decentralized healthcare environments. Their low cost, portability, simple fabrication, and limited equipment requirements support access to testing where laboratory infrastructure may be unavailable. The platforms can address biological and chemical targets, including biomarkers, pathogens, and environmental contaminants.
Bioengineering applies these platforms to the design of accessible tests for diverse targets and settings. Researchers can combine porous-paper transport, patterned fluid pathways, and embedded reagents to create devices for biomarkers, pathogens, or environmental contaminants. The resulting systems support point-of-care testing by linking material design with rapid signal generation and practical deployment.